PXD050008

PXD050008 is an original dataset announced via ProteomeXchange.

Title	human amygdala CS-56 enrichment LC-MS/MS
Description	Experience-dependent learning depends on synaptic plasticity. Recent findings show that effective integration of novel salient information requires coordinated processes of homo- and hetero- synaptic plasticity onto neighboring dendritic branches. In this work, we hypothesized that activity-dependent remodeling of the peri-synaptic extracellular matrix (ECM) contributes to this mechanism. We show that clusters of the peri-synaptic ECM proteoglycans, containing 6- and 2,6-sulfated chondroitin sulfates recognized by CS56 antibody, emerge in response to sensory stimuli, showing temporal and spatial coincidence with dendritic spine plasticity. CS56 co-immunoprecipitation of synaptosomal proteins identified several CS56-carrying/binding synaptic molecules that are implicated in Ca2+ signaling, vesicles cycling and AMPA-receptor exocytosis, thus suggesting their pivotal role in long-term potentiation (LTP). Finally, we demonstrated that the attenuation of CS56 glycoepitopes in the CA1 hippocampal region, through the depletion of versican as one of its main carriers, impairs LTP and object location memory in adult mice. These findings show that specific ECM glycans regulates the molecular mechanisms underlying induction and consolidation of synaptic plasticity, confirming that experience-dependent refinement of the brain ECM plays a critical role in learning and memory.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:56.708.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sinead O'Donovan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-21 08:48:02	ID requested
1	2024-05-10 07:06:22	announced
⏵ 2	2024-10-22 06:38:57	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2024.114112;

Chelini G, Mirzapourdelavar H, Durning P, Baidoe-Ansah D, Sethi MK, O'Donovan SM, Klengel T, Balasco L, Berciu C, Boyer-Boiteau A, McCullumsmith R, Ressler KJ, Zaia J, Bozzi Y, Dityatev A, Berretta S, Focal clusters of peri-synaptic matrix contribute to activity-dependent plasticity and memory in mice. Cell Rep, 43(5):114112(2024) [pubmed]

submitter keyword: Human, postmortem, amygdala, immunoprecipitation,CS56

Sinead O'Donovan
contact affiliation	Department of Neurosciences, University of Toledo
contact email	sinead.odonovan@utoledo.edu
lab head
Sinead O'Donovan
contact affiliation	University of Toledo
contact email	sinead.odonovan@utoledo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD050008

PRIDE project URI

• PRIDE
  1. PXD050008
     1. Label: PRIDE project
     2. Name: human amygdala CS-56 enrichment LC-MS/MS