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PXD050008

PXD050008 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlehuman amygdala CS-56 enrichment LC-MS/MS
DescriptionExperience-dependent learning depends on synaptic plasticity. Recent findings show that effective integration of novel salient information requires coordinated processes of homo- and hetero- synaptic plasticity onto neighboring dendritic branches. In this work, we hypothesized that activity-dependent remodeling of the peri-synaptic extracellular matrix (ECM) contributes to this mechanism. We show that clusters of the peri-synaptic ECM proteoglycans, containing 6- and 2,6-sulfated chondroitin sulfates recognized by CS56 antibody, emerge in response to sensory stimuli, showing temporal and spatial coincidence with dendritic spine plasticity. CS56 co-immunoprecipitation of synaptosomal proteins identified several CS56-carrying/binding synaptic molecules that are implicated in Ca2+ signaling, vesicles cycling and AMPA-receptor exocytosis, thus suggesting their pivotal role in long-term potentiation (LTP). Finally, we demonstrated that the attenuation of CS56 glycoepitopes in the CA1 hippocampal region, through the depletion of versican as one of its main carriers, impairs LTP and object location memory in adult mice. These findings show that specific ECM glycans regulates the molecular mechanisms underlying induction and consolidation of synaptic plasticity, confirming that experience-dependent refinement of the brain ECM plays a critical role in learning and memory.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:38:56.708.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSinead O'Donovan
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-21 08:48:02ID requested
12024-05-10 07:06:22announced
22024-10-22 06:38:57announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.celrep.2024.114112;
Chelini G, Mirzapourdelavar H, Durning P, Baidoe-Ansah D, Sethi MK, O'Donovan SM, Klengel T, Balasco L, Berciu C, Boyer-Boiteau A, McCullumsmith R, Ressler KJ, Zaia J, Bozzi Y, Dityatev A, Berretta S, Focal clusters of peri-synaptic matrix contribute to activity-dependent plasticity and memory in mice. Cell Rep, 43(5):114112(2024) [pubmed]
Keyword List
submitter keyword: Human, postmortem, amygdala, immunoprecipitation,CS56
Contact List
Sinead O'Donovan
contact affiliationDepartment of Neurosciences, University of Toledo
contact emailsinead.odonovan@utoledo.edu
lab head
Sinead O'Donovan
contact affiliationUniversity of Toledo
contact emailsinead.odonovan@utoledo.edu
dataset submitter
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Dataset FTP location
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