PXD050004

PXD050004 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of NKG7-proximal proteins by TurboID
Description	NKG7 is a lysosomal membrane protein whose expression is restricted to cytotoxic lymphocytes. Although the importance of NKG7 in anti-tumor immunity has been shown, little is known about the molecular function and regulation of NKG7. To elucidate NKG7 molecular function, we performed NKG7-proximity labeling using TurboID system in 293T cells, followed by mass-spectrometry. We identified vacuolar H+ ATPase (v-ATPase; lysosomal proton pump) subunit ATP6AP2 as a potential NKG7-interacting protein, and validated the interaction via exogenous coimmunoprecipitation. We further showed that NKG7 inhibits v-ATPase activity, thereby impacting lysosomal homeostasis and mTORC1 regulation at lysosomes.
HostingRepository	MassIVE
AnnounceDate	2025-01-29
AnnouncementXML	Submission_2025-01-29_08:24:24.394.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kenneth L. Johnson
SpeciesList	scientific name: Human;
ModificationList	Biotin; L-methionine sulfoxide; Carbamidomethyl
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-21 07:44:21	ID requested
⏵ 1	2025-01-29 08:24:24	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: TurboID, proximity labeling, NKG7, cytotoxic lymphocytes, lysosome

submitter keyword: TurboID, proximity labeling, NKG7, cytotoxic lymphocytes, lysosome

Contact List

Daniel Billadeau, Ph.D.
contact affiliation	Mayo Clinic, Rochester, MN
contact email	Billadeau.Daniel@mayo.edu
lab head
Kenneth L. Johnson
contact affiliation	Mayo Clinic
contact email	johnson.kenneth@mayo.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094141/