PXD049998

PXD049998 is an original dataset announced via ProteomeXchange.

Title	Altered Extracellular Vesicle-derived protein and microRNA signatures in bronchoalveolar lavage fluid from COPD patients
Description	Abstract: Chronic obstructive pulmonary disease (COPD) is a progressive lung disease for which there is no cure. Accumulating research results suggest a role for extracellular vesicles (EVs) in the pathogenesis of COPD. This study aimed to uncover the involvement of EVs and their molecular cargo in the progression of COPD by identification of EV-associated protein and microRNA (miRNA) profiles. We isolated EVs from bronchial alveolar lavage fluid (BALF) of 18 patients with COPD and 11 healthy controls by size exclusion chromatography. EV isolates were characterized by Nnanoparticle tracking analysis and protein content. Proteomic analysis revealed higher abundance of 284 proteins (log2FC > 1) and lower abun-dance of 3 proteins (log2FC <-1) in EVs derived from patients with COPD. Ingenuity pathway analysis showed that proteins enriched in COPD-associated EVs trigger inflammatory responses including neutrophil degranulation. Variances in surface receptors and ligands associated with COPD EVs suggested preferential interaction with alveolar cells. Small RNAseq analysis iden-tified higher abundance of ten miRNAs and lower abundance of one in EVs from COPD versus controls (Basemean >100, FDR<0.05). Our data indicate that the molecular composition of EVs in BALF of patients with COPD is altered compared to healthy control EVs. Several components in COPD EVs were identified that may perpetuate inflammation and alveolar tissue destruction.
HostingRepository	PRIDE
AnnounceDate	2024-06-23
AnnouncementXML	Submission_2024-06-23_02:01:51.948.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justina Clarinda Wolters
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-21 06:58:31	ID requested
⏵ 1	2024-06-23 02:01:52	announced

10.3390/cells13110945;

Bartel S, Wolters JC, Noor H, Rafie K, Fang J, Kirchner B, Nolte-T Hoen E, Pfaffl MW, Rutgers S, Timens W, van den Berge M, Hylkema MN, Altered Extracellular Vesicle-Derived Protein and microRNA Signatures in Bronchoalveolar Lavage Fluid from Patients with Chronic Obstructive Pulmonary Disease. Cells, 13(11):(2024) [pubmed]

submitter keyword: COPD, bronchoalveolar lavage,proteomics, inflammation, Extracellular Vesicles, miRNA

Justina Clarinda Wolters
contact affiliation	Department of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
contact email	j.c.wolters@umcg.nl
lab head
Justina Clarinda Wolters
contact affiliation	UMCG
contact email	j.c.wolters@umcg.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049998
     1. Label: PRIDE project
     2. Name: Altered Extracellular Vesicle-derived protein and microRNA signatures in bronchoalveolar lavage fluid from COPD patients