PXD049985

PXD049985 is an original dataset announced via ProteomeXchange.

Title	Elp1_deficency_during_early_myeloid_differentiation
Description	The replacement of microglia with myeloid cells derived from transplanted bone marrow represents a promising cell therapeutic concept for different neurological disorders. Previous studies have primarily been based on freshly harvested grafts, permitting only limited control over cells prior to transplantation. Here we demonstrate the ability of in vitro expanded hematopoietic stem and progenitor cells (cHSPCs) to engraft in the myeloid niche of the brain, and effectively replace endogenous microglia both in vivo and in vitro. Utilizing genetic editing of cHSPCs, we identify elongator acetyltransferase complex subunit 1 (Elp1; also known as Ikbkap; encoding for the protein IKAP) as an essential gene for the generation of cHSPC-derived myeloid cells (HDMCs). In accordance with its role as a subunit of the Elongator complex, loss of Elp1 in early-stage HDMCs led to upregulation of a gene module associated with the unfolded protein response.
HostingRepository	PRIDE
AnnounceDate	2026-07-18
AnnouncementXML	Submission_2026-07-18_15:06:21.167.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marius Mader
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-21 06:51:59	ID requested
⏵ 1	2026-07-18 15:06:21	announced

Dataset with its publication pending

submitter keyword: Hematopoietic cells,Elp1, Mouse, Myeloid cells, LC-MS/MS

Marius Wernig
contact affiliation	Stanford University
contact email	wernig@stanford.edu
lab head
Marius Mader
contact affiliation	Stanford University
contact email	maderm@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD049985

PRIDE project URI

• PRIDE
  1. PXD049985
     1. Label: PRIDE project
     2. Name: Elp1_deficency_during_early_myeloid_differentiation