PXD049983

PXD049983 is an original dataset announced via ProteomeXchange.

Title	Human hepatic micropeptide LC-MS/MS
Description	Considering the large number of sORFs in eukaryotic cells, micropeptides might be a long-neglected potential resource pool. However, due to the methodological limitations of micropeptide identification, tumor-related micropeptides have not been comprehensively identified, and their functional mechanisms in tumor progression remained largely uncharacterized. In the present study, we systematically identified micropeptides within HCC tissue samples based on mass spectrometry (MS) detection. By producing a high-coverage micropeptide sequencing reference library and devising an alternative ultrafiltration-concentrated tandem mass spectrometry assay, 1532 unannotated micropeptides (with no overlap with previously annotated CDSs) were successfully identified. It turned out that 81.5% of these micropeptides derived from novel ORFs and 70% of them used non-ATG start codons. Noteworthy, 5.3% of micropeptides were derived from lncRNAs, which are previously considered incapable of protein-coding. Our systematic screening of liver cancer-related micropeptides and in-depth exploration of their functions will improve our understanding of HCC pathogenesis and molecular targeted therapy.
HostingRepository	PRIDE
AnnounceDate	2025-08-16
AnnouncementXML	Submission_2025-08-16_02:24:53.548.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Linyu Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-21 06:49:50	ID requested
⏵ 1	2025-08-16 02:24:53	announced

10.1016/J.MOLCEL.2025.05.027;

submitter keyword: micropeptide， hepatocellular carcinoma

Linyu Zhu
contact affiliation	Zhejiang University
contact email	3190100927@zju.edu.cn
lab head
Linyu Zhu
contact affiliation	Zhejiang university
contact email	3190100927@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD049983

PRIDE project URI

• PRIDE
  1. PXD049983
     1. Label: PRIDE project
     2. Name: Human hepatic micropeptide LC-MS/MS