PXD049967

PXD049967 is an original dataset announced via ProteomeXchange.

Title	Aging-related Alterations in mTOR Signaling Promote Platelet Hyperreactivity and Thrombosis
Description	Isobaric labeling quantitative phospho proteomics uncovered differential site-specific protein phosphorylation within mTOR, Rho GTPase and MAPK systems in platelets from aged donors. Western blot confirmed constitutive activation of the mTOR pathway in platelets from both aged humans and mice, which was associated with increased aggregation compared to young controls. Inhibition of mTOR either with Torin 1 in aged humans, or genetic deletion in aged mice, reversed platelet hyperreactivity. Mechanistically, we noted increased levels of mitochondrial reactive oxygen species and Rac1 activation in resting platelets from aged mice, as well as increased p38 phosphorylation upstream of thromboxane generation following agonist stimulation.
HostingRepository	PRIDE
AnnounceDate	2025-05-25
AnnouncementXML	Submission_2025-05-25_08:37:23.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-20 09:10:45	ID requested
⏵ 1	2025-05-25 08:37:24	announced

10.1016/j.jtha.2024.05.025;

Portier I, Manne BK, Kosaka Y, Tolley ND, Denorme F, Babur Ö, Reddy AP, Wilmarth PA, Aslan JE, Weyrich AS, Rondina MT, Campbell RA, Aging-related alterations in mechanistic target of rapamycin signaling promote platelet hyperreactivity and thrombosis. J Thromb Haemost, 22(9):2576-2588(2024) [pubmed]

submitter keyword: platelets, phosphoproteome, thrombosis,aging, quantitative proteomics, tandem mass tag

Dr. Joe Aslan
contact affiliation	Knight Cardiovascular Institute Oregon Health & Science University Portland, Oregon USA
contact email	aslanj@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD049967

PRIDE project URI

• PRIDE
  1. PXD049967
     1. Label: PRIDE project
     2. Name: Aging-related Alterations in mTOR Signaling Promote Platelet Hyperreactivity and Thrombosis