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PXD049711

PXD049711 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Pd (II) reduction mechanisms in Bacillus megaterium Y-4 revealed by proteomic analysis
DescriptionMany studies have been conducted on microbial reduction of Pd (II) to palladium nanoparticles (PdNPs) due to the environmental friendliness, low cost and the decreased toxicity of Pd (II) ions. In this study, we investigate the reduction mechanism of Pd (II) by Bacillus megaterium Y-4 through proteomics. Our results revealed that B. megaterium Y-4 may use the endogenous electron donor (NAD(P)H) generated by nirB, tdh and fabG and reductase to reduce Pd (II) to PdNPs. The expression levels of fabG, tdh, gudB and rocG that generates NAD(P)H were further increased, and the amount of reduced PdNPs was further increased in the presence of exogenous electron donor sodium formate. Endogenous electron mediators such as quinones and flavins in B. megaterium Y-4, can further enhance Pd (II) reduction. The findings provided invaluable information regarding the reduction mechanism of Pd (II) by B. megaterium Y-4 at the proteome level.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:42:26.354.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuan Chen
SpeciesList scientific name: environmental samples
ModificationListNo PTMs are included in the dataset
InstrumentThermo Fisher Scientific instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-19 22:17:50ID requested
12024-05-24 01:09:22announced
22024-10-22 06:42:28announced2024-10-22: Updated project metadata.
Publication List
Chen Y, Wang J, Chen D, Wang B, Wu J, Liu R, Li Q, Y-4 Revealed by Proteomic Analysis. Nanomaterials (Basel), 14(6):(2024) [pubmed]
10.3390/nano14060512;
Keyword List
submitter keyword: LC-MSMS,Bacillus megaterium
Contact List
Yuan Chen
contact affiliationGuangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, China
contact email740441104@qq.com
lab head
Yuan Chen
contact affiliationInstitute of Biological and Medical Engineering, Guangdong Academy of Sciences
contact email740441104@qq.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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