PXD049711

PXD049711 is an original dataset announced via ProteomeXchange.

Title	The Pd (II) reduction mechanisms in Bacillus megaterium Y-4 revealed by proteomic analysis
Description	Many studies have been conducted on microbial reduction of Pd (II) to palladium nanoparticles (PdNPs) due to the environmental friendliness, low cost and the decreased toxicity of Pd (II) ions. In this study, we investigate the reduction mechanism of Pd (II) by Bacillus megaterium Y-4 through proteomics. Our results revealed that B. megaterium Y-4 may use the endogenous electron donor (NAD(P)H) generated by nirB, tdh and fabG and reductase to reduce Pd (II) to PdNPs. The expression levels of fabG, tdh, gudB and rocG that generates NAD(P)H were further increased, and the amount of reduced PdNPs was further increased in the presence of exogenous electron donor sodium formate. Endogenous electron mediators such as quinones and flavins in B. megaterium Y-4, can further enhance Pd (II) reduction. The findings provided invaluable information regarding the reduction mechanism of Pd (II) by B. megaterium Y-4 at the proteome level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:26.354.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuan Chen
SpeciesList	scientific name: environmental samples
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-19 22:17:50	ID requested
1	2024-05-24 01:09:22	announced
⏵ 2	2024-10-22 06:42:28	announced	2024-10-22: Updated project metadata.

Chen Y, Wang J, Chen D, Wang B, Wu J, Liu R, Li Q, Y-4 Revealed by Proteomic Analysis. Nanomaterials (Basel), 14(6):(2024) [pubmed]

10.3390/nano14060512;

submitter keyword: LC-MSMS,Bacillus megaterium

Yuan Chen
contact affiliation	Guangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, China
contact email	740441104@qq.com
lab head
Yuan Chen
contact affiliation	Institute of Biological and Medical Engineering, Guangdong Academy of Sciences
contact email	740441104@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD049711

PRIDE project URI

• PRIDE
  1. PXD049711
     1. Label: PRIDE project
     2. Name: The Pd (II) reduction mechanisms in Bacillus megaterium Y-4 revealed by proteomic analysis