PXD049705

PXD049705 is an original dataset announced via ProteomeXchange.

Title	Proxitome profiling of a viral suppressors of RNA silencing identifies the DEAD-box RNA helicase RH20 as a positive regulator of antiviral RNA interference in Nicotiana benthamiana
Description	In the prolonged arms race between viruses and their host plants, viruses usually encode viral suppressors of RNA silencing (VSR) to overcome the hosts' defense mechanism by inhibiting RNAi. Barley stripe mosaic virus (BSMV) is an RNA virus characterized by a positive single-stranded genetic structure, comprising three distinct genomic RNAs labeled as α, β, and γ. The multifunctional protein γb within BSMV’s RNAγ acts as a potent VSR, yet its underlying molecular mechanism requires further exploration. Previous studies on BSMV interacting proteins were conducted exclusively in yeast systems. We aim to identify the interacting proteins of γb under authentic conditions, specifically, the protein group interacting in the context of BSMV infection. The emerging proximity labeling (PL) technology offers several advantages, such as the identification of transiently interacting proteins, membrane proteins, and being non-toxic to plants. Consequently, this determines that PL technology may aid in resolving the challenge of identifying the interacting protein group of VSR in the context of BSMV infection.
HostingRepository	iProX
AnnounceDate	2024-02-20
AnnouncementXML	Submission_2024-10-12_00:06:47.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiyan Wen
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-19 19:32:22	ID requested
⏵ 1	2024-10-12 00:06:52	announced

Wen Z, Hu R, Pi Q, Zhang D, Duan J, Li Z, Li Q, Zhao X, Yang M, Zhao X, Liu D, Su Z, Li D, Zhang Y, DEAD-box RNA helicase RH20 positively regulates RNAi-based antiviral immunity in plants by associating with SGS3/RDR6 bodies. Plant Biotechnol J, ():(2024) [pubmed]

submitter keyword: Proxitome profiling, antiviral RNA interference, barley stripe mosaic virus, viral suppressors of RNA silencing (VSR), γb, DEAD-box RNA helicase RH20, suppressor of gene silencing 3 (SGS3), counter-defense

Yongliang Zhang
contact affiliation	China Agricultural University
contact email	cauzhangyl@cau.edu.cn
lab head
Zhiyan Wen
contact affiliation	China Agricultural University
contact email	zhiyanwen@cau.edu.cn
dataset submitter

iProX dataset URI