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PXD049705

PXD049705 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProxitome profiling of a viral suppressors of RNA silencing identifies the DEAD-box RNA helicase RH20 as a positive regulator of antiviral RNA interference in Nicotiana benthamiana
DescriptionIn the prolonged arms race between viruses and their host plants, viruses usually encode viral suppressors of RNA silencing (VSR) to overcome the hosts' defense mechanism by inhibiting RNAi. Barley stripe mosaic virus (BSMV) is an RNA virus characterized by a positive single-stranded genetic structure, comprising three distinct genomic RNAs labeled as α, β, and γ. The multifunctional protein γb within BSMV’s RNAγ acts as a potent VSR, yet its underlying molecular mechanism requires further exploration. Previous studies on BSMV interacting proteins were conducted exclusively in yeast systems. We aim to identify the interacting proteins of γb under authentic conditions, specifically, the protein group interacting in the context of BSMV infection. The emerging proximity labeling (PL) technology offers several advantages, such as the identification of transiently interacting proteins, membrane proteins, and being non-toxic to plants. Consequently, this determines that PL technology may aid in resolving the challenge of identifying the interacting protein group of VSR in the context of BSMV infection.
HostingRepositoryiProX
AnnounceDate2024-02-20
AnnouncementXMLSubmission_2024-10-12_00:06:47.183.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhiyan Wen
SpeciesList scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-19 19:32:22ID requested
12024-10-12 00:06:52announced
Publication List
Wen Z, Hu R, Pi Q, Zhang D, Duan J, Li Z, Li Q, Zhao X, Yang M, Zhao X, Liu D, Su Z, Li D, Zhang Y, DEAD-box RNA helicase RH20 positively regulates RNAi-based antiviral immunity in plants by associating with SGS3/RDR6 bodies. Plant Biotechnol J, ():(2024) [pubmed]
Keyword List
submitter keyword: Proxitome profiling, antiviral RNA interference, barley stripe mosaic virus, viral suppressors of RNA silencing (VSR), γb, DEAD-box RNA helicase RH20, suppressor of gene silencing 3 (SGS3), counter-defense
Contact List
Yongliang Zhang
contact affiliationChina Agricultural University
contact emailcauzhangyl@cau.edu.cn
lab head
Zhiyan Wen
contact affiliationChina Agricultural University
contact emailzhiyanwen@cau.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI