PXD049698

PXD049698 is an original dataset announced via ProteomeXchange.

Title	Organic molecules are involved in biomineralization by Streptomyces mirabilis
Description	The Gram-positive, filamentous soil bacterium S. mirabilis P16B-1 has been shown to tolerate environmental concentrations of over 100 mM of Ni. The extremely high metal tolerance is partly provided through biomineralization observed for both Mg bearing struvite and Ni-struvite where Ni ions replace Mg in the mineral lattice. The capacity to induce mineral formation even at some distance to the cell surface indicated involvement of secreted substances in the process. Here, we could show that metabolites as well as proteins in the cell free culture natant promote mineralization. At the same time, the precipitation process if induced physically leads to some co-precipitation which might hint at first insight into nucleation. Proteomics and metabolomics were performed in sterile culture supernatant before or after precipitation of crystals was induced by freezing and thawing to obtain candidates for co-precipitated organic molecules. Three compounds could be identified to be co-precipitated with minerals formed. However, none had an impact on macro-morphology of crystals if added as pure substance. Hence, the proteome secreted by the cells was screened, and evidence for involvement of a nickel binding protein, NikA, could be obtained.
HostingRepository	PRIDE
AnnounceDate	2026-07-07
AnnouncementXML	Submission_2026-07-07_07:33:51.026.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Streptomyces mirabilis; NCBI TaxID: NEWT:68239;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-19 12:24:21	ID requested
⏵ 1	2026-07-07 07:33:51	announced

10.1007/s12033-026-01580-3;

Costa FS, Kr, ü, ger T, Ueberschaar N, Kniemeyer O, Brakhage A, Kothe E, Organic Molecules are Involved in Ni-Struvite Biomineralization by Streptomyces mirabilis. Mol Biotechnol, ():(2026) [pubmed]

submitter keyword: struvite, biomimetic mineral formation, metabolome,Biomineralization, nickel, Streptomyces, proteome

Axel A. Brakhage
contact affiliation	Department for Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Jena, Leibniz-HKI
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD049698

PRIDE project URI

• PRIDE
  1. PXD049698
     1. Label: PRIDE project
     2. Name: Organic molecules are involved in biomineralization by Streptomyces mirabilis