PXD049693

PXD049693 is an original dataset announced via ProteomeXchange.

Title	Targeted protein editing with an antibody-based system
Description	The chemical modification of proteins is one of the major mechanisms used to regulate the properties and functions of these macromolecules in the cell. It is therefore of great interest to develop tools to exploit this type of modifications for applications in molecular biology, medicine and biotechnology. Here we present a method of using antibodies to perform post-translational covalent modifications of endogenous proteins in complex environments by exploiting proximity-driven chemistry. The method is based on the ability of antibodies to hold a weakly reactive group close to its intended site of reaction by binding the target protein on a nearby epitope. We characterise this approach by modifying the green fluorescent protein in increasingly complex environments, and illustrate its applicability by targeting the disease-associated protein beta-2 microglobulin. This dataset contains proteomics data for the identification of the modification site(s) on PEABS targets.
HostingRepository	PRIDE
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-07_16:07:21.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oded Rimon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Aequorea victoria; NCBI TaxID: 6100;
ModificationList	biotinylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-19 09:10:35	ID requested
⏵ 1	2025-09-07 16:07:22	announced

Rimon O, Konc J, Č, ernauskien, ė I, Ali M, Roy Chowdhury V, Sormanni P, Bernardes GJL, Vendruscolo M, Targeted Protein Modification with an Antibody-Based System. ACS Cent Sci, 11(8):1364-1376(2025) [pubmed]

10.1021/acscentsci.5c00651;

submitter keyword: antibody, post-translational modification, targeted,PEABS, nanobody, b2m, GFP, TPTM, beta-2 microglobulin

Michele Vendruscolo
contact affiliation	Centre for Misfolding Diseases, Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, United Kingdom
contact email	mv245@cam.ac.uk
lab head
Oded Rimon
contact affiliation	University of Cambridge
contact email	or272@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD049693

PRIDE project URI

• PRIDE
  1. PXD049693
     1. Label: PRIDE project
     2. Name: Targeted protein editing with an antibody-based system