PXD049467
PXD049467 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mi-2β promotes immune evasion in melanoma by activating EZH2 methylation |
| Description | For identification of Mi-2β-interacting proteins, B16F10 cells (1 × 108) were collected and washed three times with PBS and lysed in lysis buffer on ice for 30 min. Cell lysates were collected and then isolated over night by anti-Mi-2β magnetic agarose beads. The anti-Mi-2β magnetic agarose beads were collected and washed three times with PBS. The prepared protein samples were incubated with sample buffer for 15 min at 100 °C, and then separated by SDS-PAGE (10%). The gel was immersed in staining solution (0.3% Coomassie blue, 45% methanol, 10% glacial acetic acid and 45% dH2O) on shaker for 30 min, followed by incubation in destaining solution (20% methanol, 10% glacial acetic acid, and 70% dH2O) on the shaker overnight. The bands were excised and sent to Biological Mass Spectrometry Facility of Shanghai Applied Protein Technology Co., Ltd for protein identification. |
| HostingRepository | iProX |
| AnnounceDate | 2024-02-18 |
| AnnouncementXML | Submission_2024-03-18_19:08:24.863.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Cang Li |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ; Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-02-18 18:03:23 | ID requested | |
| 1 | 2024-02-18 18:03:51 | announced | |
| ⏵ 2 | 2024-03-18 19:08:25 | announced | 2024-03-19: Update information. |
Publication List
| Li C, Wang Z, Yao L, Lin X, Jian Y, Li Y, Zhang J, Shao J, Tran PD, Hagman JR, Cao M, Cong Y, Li HY, Goding CR, Xu ZX, Liao X, Miao X, Cui R, promotes immune evasion in melanoma by activating EZH2 methylation. Nat Commun, 15(1):2163(2024) [pubmed] |
Keyword List
| submitter keyword: CHD4, EZH2 |
Contact List
| Cang Li | |
|---|---|
| contact affiliation | The Second Affiliated Hospital, Zhejiang University |
| contact email | licang@zju.edu.cn |
| lab head | |
| Cang Li | |
| contact affiliation | The Second Affiliated Hospital, Zhejiang University |
| contact email | licang@zju.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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