PXD049453
PXD049453 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The RNF214-TEAD-YAP signaling axis promotes hepatocellular carcinoma progression via TEAD ubiquitylation |
Description | We established an APEX proximity labeling strategy in coupled with mass spectrometry to identify interacting proteins of RNF214. In this approach, we first fused an engineered ascorbate peroxidase (APEX2) to either N-terminus or C-terminus of RNF214, expressed these two fusion proteins in HLF, an HCC cell line, near the endogenous level, and generated short-lived radicals around the APEX2-RNF214 fusion proteins to label biotin on nearby interactive proteins by adding hydrogen peroxide (H2O2) and biotin-phenol (also called biotin-tyramide) transiently. Biotinylated proteins were then isolated using Streptavidin resin for protein identification by mass spectrometry. |
HostingRepository | iProX |
AnnounceDate | 2024-02-18 |
AnnouncementXML | Submission_2024-02-17_23:13:04.251.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mengjia Lin |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-02-17 23:12:41 | ID requested | |
⏵ 1 | 2024-02-17 23:13:04 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: APEX2 |
Contact List
Jianping Jin | |
---|---|
contact affiliation | Life Sciences Institute, Zhejiang University |
contact email | jianping_jin@zju.edu.cn |
lab head | |
Mengjia Lin | |
contact affiliation | Life Sciences Institute, Zhejiang University |
contact email | linmengjia9@zju.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |