PXD049432

PXD049432 is an original dataset announced via ProteomeXchange.

Title	Chlamydia trachomatis effector Tri1 interacts with TRAF7 to displace native TRAF7 interactors
Description	Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infections in the US and preventable blindness worldwide. This obligate intracellular pathogen replicates within a membrane-bound inclusion, but how it acquires nutrients from the host while avoiding detection by the innate immune system is incompletely understood. Chlamydia accomplishes this in part through the translocation of a unique set of effectors into the inclusion membrane, the inclusion membrane proteins (Incs). Incs are ideally poised at the host-pathogen interface to reprogram host signaling by redirecting proteins or organelles to the inclusion. Using a combination of co-affinity purification, immunofluorescence confocal imaging, and proteomics, we characterize the interaction between an early-expressed Inc of unknown function, Tri1, and tumor necrosis factor receptor associated factor 7 (TRAF7). TRAF7 is a multi-domain protein with a RING finger ubiquitin ligase domain and a C-terminal WD40 domain, the latter is mutated in a subset of tumors. TRAF7 regulates several innate immune signaling pathways associated with C. trachomatis infection. We demonstrate that Tri1 and TRAF7 specifically interact during infection and that TRAF7 is recruited to the inclusion. We further show that the predicted coiled-coil domain Tri1 is necessary and sufficient to interact with the TRAF7 WD40 domain. Finally, we demonstrate that Tri1 displaces native TRAF7 binding partners, MEKK2 and MEKK3. Together, our results suggest that by displacing the TRAF7 native binding partners MEKK2 and/or MEKK3, Tri1 has the capacity to alter TRAF7 signaling during infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:10.236.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danielle Swaney
SpeciesList	scientific name: Chlamydia trachomatis; NCBI TaxID: 813; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-16 12:57:23	ID requested
1	2024-05-04 20:58:38	announced
⏵ 2	2024-10-22 06:38:10	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Chlamydia trachomatis,APMS

Danielle Swaney
contact affiliation	UCSF
contact email	daniswan@gmail.com
lab head
Danielle Swaney
contact affiliation	UCSF
contact email	daniswan@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049432
     1. Label: PRIDE project
     2. Name: Chlamydia trachomatis effector Tri1 interacts with TRAF7 to displace native TRAF7 interactors