PXD049428 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Unraveling the Molecular Mechanism of Biofilm Formation by Rhodanobacter under Environmental Stressors |
Description | Bacteria are known to adhere to surfaces via self-produced extracellular polymeric substances organized as biofilms. In subsurface areas with low oxygen, limited nutrients, and toxic contaminants, biofilms are crucial for microbial survival and persistence. However, the relationship between biofilm formation and survival in such environments is not well-documented. At the Oak Ridge Reservation Field Research Center (ORRFRC), we observed a high abundance of Rhodanobacter species in conditions with elevated nitrate, metals, organics, and nitric acid. This study investigated the role of biofilm formation in their survival and the underlying molecular mechanisms in diverse geochemical niches. We examined sixteen phylogenetically diverse Rhodanobacter strains for biofilm formation under varying nutrient, pH, and nitrate conditions. Our findings indicate that biofilm formation is a strain-specific phenotype, correlating with environmental stresses, especially in low pH and nitrate conditions. Comparative genomic analysis revealed unique traits in the high biofilm-forming FW021-MT20 strain, such as the absence of flagella and chemotaxis genes and the presence of unique secretion system VI genes, as supported by pangenomic results. Additional tests on biofilm formation in response to field-relevant metals highlighted increased biofilm formation under aluminum stress in strains typically exhibiting weaker biofilm capabilities. Further investigation using RB-Tnseq, proteomics, and TEM indicated flagellar loss under aluminum stress, linked to increased cyclic AMP and di-GMP levels. Our results shed light on the adaptive strategies of Rhodanobacter strains in subsurface environments, suggesting genetic factors linked to biofilm formation and metal stress tolerance, thereby enhancing our understanding of microbial survival under environmental stress. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-08 |
AnnouncementXML | Submission_2025-03-07_19:56:27.097.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christopher Petzold |
SpeciesList | scientific name: Rhodanobacter sp.; NCBI TaxID: 1883446; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-16 10:38:35 | ID requested | |
⏵ 1 | 2025-03-07 19:56:28 | announced | |
Publication List
10.1093/ismejo/wrae151; |
Chen M, Trotter VV, Walian PJ, Chen Y, Lopez R, Lui LM, Nielsen TN, Malana RG, Thorgersen MP, Hendrickson AJ, Carion H, Deutschbauer AM, Petzold CJ, Smith HJ, Arkin AP, Adams MWW, Fields MW, Chakraborty R, Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of Rhodanobacter under environmental stress. ISME J, 18(1):(2024) [pubmed] |
Keyword List
submitter keyword: stress response,Biofilm, Rhodanobacter |
Contact List
Christopher Petzold |
contact affiliation | Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720 |
contact email | cjpetzold@lbl.gov |
lab head | |
Christopher Petzold |
contact affiliation | Lawrence Berkeley National Laboratory |
contact email | cjpetzold@lbl.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049428
- Label: PRIDE project
- Name: Unraveling the Molecular Mechanism of Biofilm Formation by Rhodanobacter under Environmental Stressors