PXD049425

PXD049425 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of the interactome of a homologue of the DEAD-box RNA helicase eIF4A and its mutant version (DQAD) from Trypanosoma brucei
Description	eIF4A is a RNA helicase considered the prototype for the DEAD-box family and it is required in different events associated with the metabolism of RNAs, with the DEAD motif important to its ATPase and helicase activities. Previous studies have indicated that EIF4AI is the functional homolog working on protein synthesis in trypanosomatids. In order to investigate in more detail the interactome of the T. brucei EIF4AI in the translation context, as well as the importance of the DEAD motif for the main protein-protein interactions, we performed co-immunoprecipitation assays of HA-tagged EIF4AI and a version with a mutation on the DEAD motif (DQAD), combined to mass spectrometry analysis. The analysis confirmed a specific association of EIF4AI with an eIF4F complex based on EIF4E4/EIF4G3 subunits, and other translation factors. A differential pattern of association was seen for the mutant protein, indicating that the DEAD motif might be important to the specific eIF4F assembly in T. brucei. This study helped to clarify functional aspects of the EIF4AI helicase during Trypanosoma mRNA translation/metabolism.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:07:09.925.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludmila Arruda de Assis
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-16 06:48:51	ID requested
⏵ 1	2025-05-25 16:07:10	announced

Moura DMN, Soares AL, da Silva A, Ribeiro JLAB, Sunter JD, Assis LA, Carrington M, de Melo Neto OP, Distinct modes of interaction within eIF4F-like complexes and susceptibility to the RocA inhibitor for the Trypanosoma brucei EIF4AI translation initiation factor. PLoS One, 20(5):e0322812(2025) [pubmed]

10.1371/journal.pone.0322812;

submitter keyword: eIF4F, translation initiation,eIF4A, RNA helicase

Osvaldo Pompílio de Melo Neto
contact affiliation	Instituto Aggeu Magalhães - FIOCRUZ, Recife, PE, Brazil
contact email	osvaldo.pompilio@fiocruz.br
lab head
Ludmila Arruda de Assis
contact affiliation	Instituto Aggeu Magalhães - Fiocruz Pernambuco
contact email	ludmila18arruda@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD049425

PRIDE project URI

• PRIDE
  1. PXD049425
     1. Label: PRIDE project
     2. Name: Comparative analysis of the interactome of a homologue of the DEAD-box RNA helicase eIF4A and its mutant version (DQAD) from Trypanosoma brucei