PXD049411

PXD049411 is an original dataset announced via ProteomeXchange.

Title	Pre-transfusion storage alters the proteostasis function and capacity to circulate of a morphologically- and metabolically-altered red cell subpopulation, but preserves other red cells
Description	This study aimed to provide a characterization of morphologically-altered red blood cells (RBCs) and to compare their properties to those of long-stored morphologically normal RBCs and to short-stored RBCs. For proteomics experiments, RBC concentrates stored in blood bank conditions were submitted to a CFSE staining protocol that allows specific sorting of morphologically-altered (CFSEhigh) and normal (CFSElow) RBC subpopulations. Proteome of erythrocytes and ghosts were analyzed at day 3-10 (short-stored, CFSElow RBC subpopulation) and day 40-44 (long-stored, CFSEhigh and CFSElow RBC subpopulations) of storage by a label free quantification approach.
HostingRepository	PRIDE
AnnounceDate	2025-04-02
AnnouncementXML	Submission_2025-04-02_15:04:33.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilie-Fleur GAUTIER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-15 12:02:00	ID requested
⏵ 1	2025-04-02 15:04:33	announced

10.1172/JCI183099;

submitter keyword: proteomics, proteasome activity, red blood cells,Red cell storage, LFQ, CFSE staining, spleen filtration, red cell morphology, proteostasis

Dr Pascal Amireault
contact affiliation	Université Paris Cité et Université des Antilles, INSERM U1134, BIGR, Paris, France, Institut Imagine, Laboratory of Cellular and Molecular Mechanisms of Hematological Disorders and Therapeutic Implications, INSERM U1163, Paris, France
contact email	pascal.amireault@inserm.fr
lab head
Emilie-Fleur GAUTIER
contact affiliation	Plateforme Proteomique 3P5, Université Paris Descartes, Inserm U1016, Institut Cochin, Cnrs UMR8104, France
contact email	ef.gautier.upd@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD049411

PRIDE project URI

• PRIDE
  1. PXD049411
     1. Label: PRIDE project
     2. Name: Pre-transfusion storage alters the proteostasis function and capacity to circulate of a morphologically- and metabolically-altered red cell subpopulation, but preserves other red cells