PXD049401

PXD049401 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Nuclear and total proteins in THP-1 cell differentiation
Description	THP-1 monocyte-like suspension cells can be differentiated into macrophage-like adherent cells by TPA (PMA) treatment. Proteomic analysis was carried out on (i) normal THP-1 cells, (ii) THP-1 cells in which KPNA1 (importin-alpha5) was knocked down or not by siRNA, and (iii) THP-1 TetOn-KPNA2 (importin-alpha1) cells induced or not by Dox. Total and nuclear proteins were prepared from both the monocyte-like and macrophage-like cells and quantified by a label-free method.
HostingRepository	jPOST
AnnounceDate	2025-02-15
AnnouncementXML	Submission_2025-03-12_19:02:09.461.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Makoto Kimura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-15 06:00:11	ID requested
1	2025-02-14 07:00:05	announced
⏵ 2	2025-03-12 19:02:10	announced	2025-03-13: Updated PubMed.

Publication List

Kimura M, Ogawa Y, Motohashi S, Imamoto N, Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation. FEBS Lett, 599(6):813-827(2025) [pubmed]

Kimura M, Ogawa Y, Motohashi S, Imamoto N, Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation. FEBS Lett, 599(6):813-827(2025) [pubmed]

Keyword List

submitter keyword: THP-1, monocyte, macrophage, nuclear protein, LFQ

submitter keyword: THP-1, monocyte, macrophage, nuclear protein, LFQ

Contact List

Makoto Kimura
lab head
Makoto Kimura
contact affiliation	RIKEN
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002504/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002504/