PXD049401
PXD049401 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nuclear and total proteins in THP-1 cell differentiation |
Description | THP-1 monocyte-like suspension cells can be differentiated into macrophage-like adherent cells by TPA (PMA) treatment. Proteomic analysis was carried out on (i) normal THP-1 cells, (ii) THP-1 cells in which KPNA1 (importin-alpha5) was knocked down or not by siRNA, and (iii) THP-1 TetOn-KPNA2 (importin-alpha1) cells induced or not by Dox. Total and nuclear proteins were prepared from both the monocyte-like and macrophage-like cells and quantified by a label-free method. |
HostingRepository | jPOST |
AnnounceDate | 2025-02-15 |
AnnouncementXML | Submission_2025-03-12_19:02:09.461.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Makoto Kimura |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-02-15 06:00:11 | ID requested | |
1 | 2025-02-14 07:00:05 | announced | |
⏵ 2 | 2025-03-12 19:02:10 | announced | 2025-03-13: Updated PubMed. |
Publication List
Kimura M, Ogawa Y, Motohashi S, Imamoto N, Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation. FEBS Lett, 599(6):813-827(2025) [pubmed] |
Keyword List
submitter keyword: THP-1, monocyte, macrophage, nuclear protein, LFQ |
Contact List
Makoto Kimura | |
---|---|
lab head | |
Makoto Kimura | |
contact affiliation | RIKEN |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002504/ |