PXD049376

PXD049376 is an original dataset announced via ProteomeXchange.

Title	BODIPY-Patatin assay proteomics
Description	The potato protein patatin embeds bioactive peptides that require targeted hydrolysis to be released as promising food additives. This study presents a patatin-specific protease assay for assessing a wide range of protease activities in high-throughput format. Conjugating patatin to the amine reactive fluorogenic BODIPY FL dye provided a stable protease substrate with efficient homo-FRET quenching at a low degree (7-8) of labeling. Compared to commercial BODIPY-casein, BODIPY-patatin provided higher fluorescence enhancement (by de-quenching) at high protease concentrations, while the sensitivity was generally comparable for both highly specific (e.g. Trypsin) and industrial relevant proteases (e.g. Alcalase and Neutrase) at low doses. For Chymotrypsin, BODIPY-patatin provided a 39% response improvement at 5 ng dose. Peptide-centric mass spectrometry analyses identified several BODIPY-labeling sites with varying occupancies in patatin, indicating heterogenous labeling under the applied conjugation conditions. BODIPY-labeled patatin complements commercial BODIPY-labeled casein as a globular, plant-based alternative for screening of proteolytic activity.
HostingRepository	PRIDE
AnnounceDate	2025-10-22
AnnouncementXML	Submission_2025-10-22_05:20:34.105.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049376
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Simon Gregersen Echers
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: NEWT:4113;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-14 07:25:57	ID requested
⏵ 1	2025-10-22 05:20:34	announced

10.6019/PXD049376;

10.1016/J.FOODCHEM.2024.141105;

submitter keyword: Potato, BODIPY-FL labeling, Q Exactive HF, patatin, site-specific modification enrichment

Simon Gregersen Echers
contact affiliation	Department of Chemistry and Bioscience Aalborg University Denmark
contact email	sgr@bio.aau.dk
lab head
Simon Gregersen Echers
contact affiliation	Department of Chemistry & Bioscience Aalborg University
contact email	sgr@bio.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049376
     1. Label: PRIDE project
     2. Name: BODIPY-Patatin assay proteomics