PXD049375 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A germline PAF1 paralog complex ensures cell type-specific gene expression (proximity labeling data) |
Description | Animal germline development and fertility relies on paralogs of general transcription factors that recruit RNA polymerase II to ensure cell type-specific gene expression. It remains unclear whether gene expression processes downstream of such paralog-based transcription is distinct from that of canonical RNA polymerase II genes. In Drosophila, the testis-specific TBP-associated factors (tTAFs) activate over a thousand spermatocyte-specific gene promoters to enable meiosis and germ cell differentiation. Here we show that efficient termination of tTAF-activated transcription requires a testis-specific Polymerase Associated Factor 1 Complex (tPAF) composed of paralogs of canonical Polymerase Associated Factor 1 Complex (PAF1C) proteins. Defective transcription termination in tPAF mutants causes aberrant expression of hundreds of downstream genes due to read-in transcription, compromising cell type-specific gene expression in spermatocytes. Consistently, tPAF is required for the segregation of meiotic chromosomes, sperm individualisation, and male fertility. Comparative in vivo proximity labeling assays of tPAF and PAF1C showed tPAF-specific association with tTAF as well as connections to central RNA polymerase II termination factors. Our study uncovers transcription termination as a developmentally regulated process required for cell type-specific gene expression. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-13 |
AnnouncementXML | Submission_2024-04-13_12:59:53.390.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Ebert Andersen |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | biotinylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-14 07:07:27 | ID requested | |
⏵ 1 | 2024-04-13 12:59:53 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proximity labeling, TurboID, Paf1 complex, germline, transcription termination, cell-type identity, paralogs |
Contact List
Peter Andersen |
contact affiliation | Deparment of Molecular Biology and Genetics Aarhus University, Denmark |
contact email | pra@mbg.au.dk |
lab head | |
Peter Ebert Andersen |
contact affiliation | Department of Molecular Biology and Genetics, Aarhus University |
contact email | pra@mbg.au.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049375
- Label: PRIDE project
- Name: A germline PAF1 paralog complex ensures cell type-specific gene expression (proximity labeling data)