PXD049371

PXD049371 is an original dataset announced via ProteomeXchange.

Title	MS-based proteomic characterization of assembled microtubules
Description	Microtubules are dynamic polymers that interconvert between phases of growth and shrinkage, yet they somehow provide lasting structural stability to cells. Growth involves hydrolysis of GTP-tubulin to GDP-tubulin, which releases energy that is stored within the microtubule lattice and destabilizes it; a GTP cap at microtubule ends is thought to prevent GDP subunits from rapidly dissociating and causing catastrophe. We show that GDP-tubulin, usually considered as an inactive tubulin species, can itself assemble into microtubules preferentially at the minus end, and promote persistent growth. We characterized by mass spectrometry-based proteomics the protein content of tubulin purified from bovine brain (Total) and of microtubules grown from stable GMPCPP seeds in the presence of either GDP-tubulin or GTP-tubulin.
HostingRepository	PRIDE
AnnounceDate	2024-04-30
AnnouncementXML	Submission_2024-04-30_09:18:00.997.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049371
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-14 06:20:03	ID requested
⏵ 1	2024-04-30 09:18:01	announced

10.6019/PXD049371;

submitter keyword: Microtubules, tubulin, purification, in-vitro assembly, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049371
     1. Label: PRIDE project
     2. Name: MS-based proteomic characterization of assembled microtubules