PXD049370

PXD049370 is an original dataset announced via ProteomeXchange.

Title	The S4-domain containing protein YlmH is involved in ribosome-associated quality control in Bacillus subtilis
Description	By taking advantage of the strong genetic interactions between trans-translation and other ribosome rescue systems, we have employed a transposon sequencing (Tn-Seq) to identify potential novel rescue factors in Bacillus subtilis. In addition to the identification the ArfA-type rescue factor BrfA, as well as the RQC elongation actors RqcP and RqcH, our Tn-Seq screen led to the identification of YlmH, a poorly characterized S4-domain-containing protein, as a potential RQC factor. Binding of YlmH to 50S ribosomal subunit was confirmed by proteomics approach.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:58:27.346.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049370
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tanel Tenson
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-14 05:53:04	ID requested
1	2024-09-16 05:13:54	announced
⏵ 2	2024-10-22 06:58:28	announced	2024-10-22: Updated project metadata.

Takada H, Paternoga H, Fujiwara K, Nakamoto JA, Park EN, Dimitrova-Paternoga L, Beckert B, Saarma M, Tenson T, Buskirk AR, Atkinson GC, Chiba S, Wilson DN, Hauryliuk V, A role for the S4-domain containing protein YlmH in ribosome-associated quality control in Bacillus subtilis. Nucleic Acids Res, 52(14):8483-8499(2024) [pubmed]

10.6019/PXD049370;

10.1093/nar/gkae399;

submitter keyword: YlmH,translation, quality control

Vasili Hauryliuk
contact affiliation	Lund University
contact email	vasili.hauryliuk@med.lu.se
lab head
Tanel Tenson
contact affiliation	University of Tartu
contact email	tanel.tenson@ut.ee
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD049370

PRIDE project URI

• PRIDE
  1. PXD049370
     1. Label: PRIDE project
     2. Name: The S4-domain containing protein YlmH is involved in ribosome-associated quality control in Bacillus subtilis