PXD049355 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bioresponsive pseudoGlucosinolates (psGSLs) release Isothiocyanates (ITCs) in the Presence of Nitroreductases |
| Description | Glucosinolates (GSLs) are secondary metabolites produced as part of an herbivore defence system in plants of the order Brassicales. GSLs release isothiocyanates (ITCs) upon activation by the myrosinase. Beyond their herbivore feeding deterrent properties, these ITCs have multiple interesting bioactivities. However, their release is limited by the presence of myrosinase. Here, we report the concept of pseudoglucosinolates (psGSLs) hijacking the natural release mechanism of GSLs for the release of ITCs and adapting it to nitroreductase as triggering enzymes. We provide the proof-of-concept for nitroreductase-responsive psGSLs and demonstrate their potential for peptide labelling and ITC-prodrug approaches. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-06_16:10:47.710.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Stephan Hacker |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: NEWT:1280; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-02-13 07:47:08 | ID requested | |
| ⏵ 1 | 2026-09-06 16:10:48 | announced | |
Publication List
| Jimidar CC, Ganskow CSG, Kagho MD, Chakrabarti A, Wiese L, Zollo M, Beutling U, Cesar LC, Morud J, Bugaj K, Br, รถ, nstrup M, Sieber SA, Hacker SM, Klahn P, Bioresponsive pseudoGlucosinolates (psGSLs) Release Isothiocyanates (ITCs) in the Presence of Nitroreductases. Chemistry, 32(25):e71012(2026) [pubmed] |
| 10.1002/chem.71012; |
Keyword List
| submitter keyword: isothiocyanates, artificial glucosinolates, bioresponsive protein labelling,pseudoglucosinolates, prodrug |
Contact List
| Stephan Hacker |
|---|
| contact affiliation | Department of Molecular Physiology, Leiden Institute of Chemistry, Leiden University |
| contact email | s.m.hacker@lic.leidenuniv.nl |
| lab head | |
| Stephan Hacker |
|---|
| contact affiliation | Leiden University |
| contact email | s.m.hacker@lic.leidenuniv.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD049355 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049355
- Label: PRIDE project
- Name: Bioresponsive pseudoGlucosinolates (psGSLs) release Isothiocyanates (ITCs) in the Presence of Nitroreductases
