PXD049343

PXD049343 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis Reveals the Major Proteins and Pathways that Mediate the Effect of 17-β-Estradiol in Cell Division and Apoptosis in Breast Cancer MCF7 Cells
Description	Despite extensive research, the genes/proteins and biological pathways responsible for the physiological effects of estrogen remain elusive. In this study, we employed a proteomic method to determine the effect of estrogen on global protein expression in breast cancer MCF7 cells. The expression of 77 cytoplasmic, 74 nuclear, and 81 membrane proteins was significantly altered by 17-β-estradiol (E2). Protein enrichment analyses of the quantified proteins revealed that E2 stimulated cell division primarily by promoting the G1 to S phase transition and advancing the G2/M checkpoint. Functional annotation analyses of the E2-regulated proteins indicated that these proteins promoted cell division primarily through enhancing protein nuclear import, protein translation, reducing mRNA degradation, and increasing protein folding. Interestingly, many of the E2-upregulated proteins contained the HEAT, KH, and RRM domains. The effect of E2 on cell survival was complex, as it could simultaneously enhance and inhibit apoptosis. E2 enhanced apoptosis by promoting cytochrome c release from mitochondria and inhibited apoptosis by activating the PI3K/AKT/mTOR signaling pathway. Treatment of MCF7 cells with E2 and the PI3K inhibitor Ly294002 significantly enhanced apoptosis compared to the cells treated with E2 alone. Our results suggest that combining estrogen with a PI3K inhibitor could be a promising strategy for treating ERα-positive breast cancer.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:09:29.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuchun Du
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-12 15:03:00	ID requested
⏵ 1	2025-05-06 13:09:30	announced

10.1021/acs.jproteome.4c00102;

Zhou Z, Sicairos B, Zhou J, Du Y, -Estradiol in Cell Division and Apoptosis in Breast Cancer MCF7 Cells. J Proteome Res, 23(11):4835-4848(2024) [pubmed]

submitter keyword: estrogen, breast cancer, proteome profiling,Quantitative proteomics, cell division, SILAC, apoptosis.

Yuchun Du
contact affiliation	University of Arkansas
contact email	ydu@uark.edu
lab head
Yuchun Du
contact affiliation	University of Arkansas
contact email	ydu@uark.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD049343

PRIDE project URI

• PRIDE
  1. PXD049343
     1. Label: PRIDE project
     2. Name: Proteomic Analysis Reveals the Major Proteins and Pathways that Mediate the Effect of 17-β-Estradiol in Cell Division and Apoptosis in Breast Cancer MCF7 Cells