PXD049341

PXD049341 is an original dataset announced via ProteomeXchange.

Title	Function of CK2 selectively affects the endoplasmic reticulum and the Golgi apparatus in mTOR-mediated autophagy induction.
Description	Selective autophagy of the endoplasmic reticulum, known as ER-phagy, is essential to maintain ER homeostasis. We recently showed that members of the autophagy receptor family FAM134 are regulated by phosphorylation-dependent ubiquitination. In an unbiased screen we had identified several kinases downstream of mTOR with profound impact on ER-phagy flux, upon them ATR and CK2. Inhibition of CK2 by SGC-CK2-1 prevented regulatory ubiquitination of FAM134B and FAM134C upon autophagy activation as well as the formation of high-density FAM134B- and FAM134C-clusters. Here we report on additional resource data of global proteomics upon CK2 and ATR inhibition, respectively. Our data suggests that the function of CK2 is mainly limited to the ER / ER-phagy and Golgi, while ATR inhibition by VE-822 affects the vast majority of organelles / selective autophagy pathways.
HostingRepository	PRIDE
AnnounceDate	2025-03-07
AnnouncementXML	Submission_2025-03-07_03:11:49.264.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pablo Sanz Martinez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-12 09:51:12	ID requested
⏵ 1	2025-03-07 03:11:50	announced

10.1080/15548627.2024.2395725;

Sanz-Martinez P, Berkane R, Stolz A, Function of CSNK2/CK2 selectively affects the endoplasmic reticulum and the Golgi apparatus in mtor-mediated autophagy induction. Autophagy, 21(2):480-486(2025) [pubmed]

submitter keyword: Human, Multiplexed, LC-MS,TMTpro 18plex,GPRC,Compounds

Alexandra Stolz
contact affiliation	Goethe University, Institute for Biochemistry II, Frankfurt am Main
contact email	stolz@em.uni-frankfurt.de
lab head
Pablo Sanz Martinez
contact affiliation	PhD student
contact email	P.SanzMartinez@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD049341

PRIDE project URI

• PRIDE
  1. PXD049341
     1. Label: PRIDE project
     2. Name: Function of CK2 selectively affects the endoplasmic reticulum and the Golgi apparatus in mTOR-mediated autophagy induction.