PXD049326

PXD049326 is an original dataset announced via ProteomeXchange.

Title	Automated high-throughput biological sex identification from archaeological human dental enamel using targeted proteomics
Description	Biological sex is a key information for archaeological and forensic studies, which can be assessed in enamel using proteomics. However, the lack of a standard approach for fast and accurate sex identification limits the reach of proteomics applications. Here, we introduce a targeted proteomics-based workflow that builds on a rapid, non-destructive, sensitive, and quantitative mass spectrometry (MS) strategy for the determination of biological sex from human dental enamel, including a R-Shiny interface for the data analysis. The method was first developed and optimised using modern teeth material and then validated on an independent set of modern deciduous teeth of known sex. The assay was then applied to archaeological material and demonstrated unprecedented specificity and scalability for molecular sex inference, with the possibility of analysing > 300 individuals in less than two days of MS measurement time, and with subsequent confident sex assignment in less than a day of data analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_06:06:39.780.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claire Koenig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-12 02:07:40	ID requested
⏵ 1	2024-10-17 06:06:40	announced

10.1021/acs.jproteome.4c00557;

Koenig C, Bortel P, Paterson RS, Rendl B, Madupe PP, Troch, é GB, Hermann NV, Mart, í, nez de Pinillos M, Martin, ó, n-Torres M, Mularczyk S, Schjellerup J, ø, rkov ML, Gerner C, Kanz F, Martinez-Val A, Cappellini E, Olsen JV, Automated High-Throughput Biological Sex Identification from Archeological Human Dental Enamel Using Targeted Proteomics. J Proteome Res, ():(2024) [pubmed]

submitter keyword: Forensics, PRM, Sex,Palaeoproteomics, Archaeology, Enamel

Jesper V. Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
contact email	jesper.olsen@cpr.ku.dk
lab head
Claire Koenig
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact email	claire.koenig@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD049326

PRIDE project URI

• PRIDE
  1. PXD049326
     1. Label: PRIDE project
     2. Name: Automated high-throughput biological sex identification from archaeological human dental enamel using targeted proteomics