PXD049312
PXD049312 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | K562 cell lysates prefractionated by various methods |
Description | K562 cell lysates were prefractionated by protein reversed-phase chromatography (Prot-RP), SDS-PAGE, and high-pH peptide RP (Pept-RP) to compare their method characteristics. The starting material was prefractionated to 10 fractions for each method. Each prefractionation method was conducted in triplicate to generate 30 raw data per method. |
HostingRepository | jPOST |
AnnounceDate | 2024-10-15 |
AnnouncementXML | Submission_2024-10-14_17:04:43.023.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shunsuke Takagi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Met-loss; Met-loss+Acetyl; Acetyl |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-02-10 02:15:28 | ID requested | |
⏵ 1 | 2024-10-14 17:04:48 | announced |
Publication List
Takagi S, Suzuki N, Ishihama Y, Revisiting Protein Reversed-Phase Chromatography for Bottom-Up Proteomics. J Proteome Res, 23(10):4704-4714(2024) [pubmed] |
Keyword List
submitter keyword: Protein prefractionation, Reversed-phase chromatography, Proteoform, Biopharmaceuticals |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Shunsuke Takagi | |
contact affiliation | Daiichi Sankyo Co., Ltd. |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002507/ |