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PXD049312

PXD049312 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleK562 cell lysates prefractionated by various methods
DescriptionK562 cell lysates were prefractionated by protein reversed-phase chromatography (Prot-RP), SDS-PAGE, and high-pH peptide RP (Pept-RP) to compare their method characteristics. The starting material was prefractionated to 10 fractions for each method. Each prefractionation method was conducted in triplicate to generate 30 raw data per method.
HostingRepositoryjPOST
AnnounceDate2024-10-15
AnnouncementXMLSubmission_2024-10-14_17:04:43.023.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShunsuke Takagi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Met-loss; Met-loss+Acetyl; Acetyl
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-10 02:15:28ID requested
12024-10-14 17:04:48announced
Publication List
Takagi S, Suzuki N, Ishihama Y, Revisiting Protein Reversed-Phase Chromatography for Bottom-Up Proteomics. J Proteome Res, 23(10):4704-4714(2024) [pubmed]
Keyword List
submitter keyword: Protein prefractionation, Reversed-phase chromatography, Proteoform, Biopharmaceuticals
Contact List
Yasushi Ishihama
lab head
Shunsuke Takagi
contact affiliationDaiichi Sankyo Co., Ltd.
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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