PXD049302 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The protective effect of docosahexaenoic acid on mitochondria in a SH-SY5Y model of rotenone-induced toxicity |
Description | Polyunsaturated fatty acids in particular omega-3 fatty acids, such as docosahexaenoic acid (DHA) are essential nutrients and components of the plasma membrane. They are involved in various processes, among them synaptic development, functionality, integrity and plasticity and are therefore thought to have general neuroprotective properties. Considerable research evidence further supports beneficial effects of omega-3 fatty acids specifically on mitochondrial structure and function through their antioxidant and anti-apoptotic properties, which makes it an attractive addition in treatment options for neurodegenerative disorders, in which mitochondrial alterations are commonly observed. However, so far precise information on the underlying protective mechanisms are still lacking. Thus in this study, we utilized the most common neuronal cell line (SH-SY5Y) and induced mitochondrial oxidative stress through the addition of rotenone. To study the potential protective effect of DHA, cells were additionally pre-treated with DHA prior rotenone administration. By combining SILAC labelling, mitochondria enrichment, and subsequent proteomic analyses, we aimed to determine the capacity of DHA to alleviate mitochondrial oxidative stress in vitro and further shed light on molecular mechanisms contributing to the proposed neuroprotective effect. Indeed we confirmed a general increase in intensity of mitochondrial proteins in DHA pre-treated cells. Furthermore, we were able to define a compensatory mechanism by which the inhibition of complex I is overcome by an increased activity of the fatty acid beta oxidation in response to DHA pre-treatment. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-24 |
AnnouncementXML | Submission_2025-02-24_02:34:00.990.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Britta Eggers |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-09 06:44:55 | ID requested | |
⏵ 1 | 2025-02-24 02:34:01 | announced | |
Publication List
10.3390/metabo15010029; |
Eggers B, Stepien J, Reker AK, Esser S, Pfeiffer K, Pawlas M, Barkovits K, Marcus K, The Protective Effect of Docosahexaenoic Acid on Mitochondria in SH-SY5Y Model of Rotenone-Induced Toxicity. Metabolites, 15(1):(2025) [pubmed] |
Keyword List
submitter keyword: DHA, proteomics,rotenone, mitochondria, SH-SY5Y |
Contact List
Katrin Marcus |
contact affiliation | Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 44801 Bochum, Germany |
contact email | katrin.marcus@rub.de |
lab head | |
Britta Eggers |
contact affiliation | Ruhr University Bochum |
contact email | britta.eggers@rub.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049302
- Label: PRIDE project
- Name: The protective effect of docosahexaenoic acid on mitochondria in a SH-SY5Y model of rotenone-induced toxicity