PXD049298 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Thermal proteome profilng of BMAL1 ligand CCM in U2OS cell lysate |
Description | The master transcription factor BMAL1-CLOCK is crucial for orchestrating rhythmic gene expression and governing circadian rhythms. Pharmacological manipulation of this central circadian regulator requires accessible protein cavities and selective compounds. By targeting BMAL1 through a resident cavity in its PAS-B domain, we demonstrate that the small-molecule Core Circadian Modulator (CCM) penetrates and substantially expands this pocket. Biochemical and cellular analyses validate CCM's target engagement selectivity, enabling direct access to BMAL1's transcriptional activities. Thermal proteome profiling of CCM in U2OS cell lysate was performed at two concentrations (50 µM and 200 µM) and compared to DMSO. The proteome integral stability assay (PISA) setup was used. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-03 |
AnnouncementXML | Submission_2025-02-03_10:03:45.351.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ludwig Bauer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-09 05:00:43 | ID requested | |
⏵ 1 | 2025-02-03 10:03:45 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Thermal proteome profiling |
Contact List
Kilian Huber |
contact affiliation | Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ, UK Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7FZ, UK |
contact email | kilian.huber@cmd.ox.ac.uk |
lab head | |
Ludwig Bauer |
contact affiliation | University of Oxford |
contact email | ludwig.bauer@st-hughs.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049298
- Label: PRIDE project
- Name: Thermal proteome profilng of BMAL1 ligand CCM in U2OS cell lysate