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PXD049298

PXD049298 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThermal proteome profilng of BMAL1 ligand CCM in U2OS cell lysate
DescriptionThe master transcription factor BMAL1-CLOCK is crucial for orchestrating rhythmic gene expression and governing circadian rhythms. Pharmacological manipulation of this central circadian regulator requires accessible protein cavities and selective compounds. By targeting BMAL1 through a resident cavity in its PAS-B domain, we demonstrate that the small-molecule Core Circadian Modulator (CCM) penetrates and substantially expands this pocket. Biochemical and cellular analyses validate CCM's target engagement selectivity, enabling direct access to BMAL1's transcriptional activities. Thermal proteome profiling of CCM in U2OS cell lysate was performed at two concentrations (50 µM and 200 µM) and compared to DMSO. The proteome integral stability assay (PISA) setup was used.
HostingRepositoryPRIDE
AnnounceDate2025-02-03
AnnouncementXMLSubmission_2025-02-03_10:03:45.351.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLudwig Bauer
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-09 05:00:43ID requested
12025-02-03 10:03:45announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Thermal proteome profiling
Contact List
Kilian Huber
contact affiliationTarget Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ, UK Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7FZ, UK
contact emailkilian.huber@cmd.ox.ac.uk
lab head
Ludwig Bauer
contact affiliationUniversity of Oxford
contact emailludwig.bauer@st-hughs.ox.ac.uk
dataset submitter
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