PXD049297

PXD049297 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Reveals Cellular Responses to Stress and Tunicamycin in CHO Cells
Description	We conducted proteomics analysis aimed at evaluating the differences between CHO cell lines expressing 2G12 and 353/11, cells were cultured using a semi-perfusion batch process in parallel. The cells were collected and pelleted on day six, four hours after medium exchange, when both cell lines had reached maximum density and exhibited sustained viability (Figure 1). One cell line exhibited high production (353/11), whereas the other one (2G12) displayed low production. To induce endoplasmic reticulum (ER) stress, 353/11 cells were cultivated separately and parallelly treated with tunicamycin (TM) at a final concentration of 1 µg/mL in fresh cultivation medium for four hours prior to harvesting. This approach aimed to establish uniform experimental conditions comparable to those of non-treated cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:48:34.863.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eldi Sulaj
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-09 04:52:03	ID requested
1	2024-07-03 02:09:34	announced
⏵ 2	2024-10-22 06:48:38	announced	2024-10-22: Updated project metadata.

10.1007/s00253-024-13223-1;

Sulaj E, Schwaigerlehner L, Sandell FL, Dohm JC, Marzban G, Kunert R, Quantitative proteomics reveals cellular responses to individual mAb expression and tunicamycin in CHO cells. Appl Microbiol Biotechnol, 108(1):381(2024) [pubmed]

submitter keyword: MS/MS, CHO,LFQ, Orbitrap Q Exactive, Proteomics

Gorji Marzban
contact affiliation	Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences Vienna
contact email	gorji.marzban@boku.ac.at
lab head
Eldi Sulaj
contact affiliation	BOKU University, Vienna
contact email	eldi.sulaj@boku.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD049297

PRIDE project URI

• PRIDE
  1. PXD049297
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Reveals Cellular Responses to Stress and Tunicamycin in CHO Cells