PXD049284

PXD049284 is an original dataset announced via ProteomeXchange.

Title	TMT-based phosphoproteomic analysis of ALK-positive patient-derived lung cancer cell lines with GUK1 phospho-null mutation and ALK TKI treatment
Description	The metabolic adaptations observed in cancer are widely recognized, yet the precise mechanisms of how these metabolic adjustments support tumor growth remain unclear. This study explores the impact of guanine nucleotide metabolism on cancer progression in ALK-driven lung cancer. We showed that GUK1 phosphorylation at Y74 augments guanine nucleotide synthesis and promotes tumor growth in ALK-driven lung cancer both in vitro and in vivo. Using 16-plex TMT-phosphoproteomics, we investigated the effect of the ALK tyrosine kinase inhibitor lorlatinib on patient-derived non-small cell lung cancer cell lines with and without GUK1 Y74F mutation, thus shedding light on signaling and metabolic pathways reliant on GUK1 phosphorylation in ALK-driven lung cancer cells.
HostingRepository	PRIDE
AnnounceDate	2025-02-03
AnnouncementXML	Submission_2025-02-03_08:10:26.643.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brandon Gassaway
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-08 09:22:21	ID requested
⏵ 1	2025-02-03 08:10:27	announced

Dataset with its publication pending

submitter keyword: ALK, GUK1, Phosphoproteomics, Non small cell lung cancer

Brandon Gassaway
contact affiliation	Steve Gygi, Harvard Medical School
contact email	brandon_gassaway@hms.harvard.edu
lab head
Brandon Gassaway
contact affiliation	Brigham Young University
contact email	brandon.gassaway@byu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049284
     1. Label: PRIDE project
     2. Name: TMT-based phosphoproteomic analysis of ALK-positive patient-derived lung cancer cell lines with GUK1 phospho-null mutation and ALK TKI treatment