PXD049265

PXD049265 is an original dataset announced via ProteomeXchange.

Title	Proteomics and Metabolomic Analyses Reveal the oxidative stress and ATP generation obstructed are major contributor to bull sperm cryoinjury
Description	An artificial vagina was used to extract fresh ejaculates from four Gaoqing bulls (4-6 years) from the Aohang farm in Shandong Province, China. To assess progressive motility, 3 uL of the fresh ejaculate was immediately evaluated through computer-assisted sperm analysis (CASA) (CASA, Nikon, Eclipse E200, 10X, Basler, acA780-75gc, SCA sperm class analyzer)). Only the ejaculates with a volume of >2.0 mL and demonstrating >70% motility were selected for the analysis. A Biladyl® extender (Minitube, GER) was used to dilute the samples in a two-step procedure. Initially, the samples were diluted with a glycerol-free formulation, which constituted 50% of the final volume estimated, and refrigerated at 5°C for 2 hours. Subsequently, the samples were diluted with a pre-cooled (5ºC) glycerol-containing formulation and refrigerated at 5ºC for 2 h. The sperm samples were frozen to a final concentration of 140–200 x 106/mL. A cooling curve of -5 ºC/min was used from 5 ºC to 4 ºC, then -3 ºC/min was used from 4 ºC to -10 ºC, then -40 ºC/min was used from -10 ºC to -100 ºC, then -20 ºC/min was used from -100 ºC to -140 ºC. All samples were stored in liquid nitrogen until further use. Sperm samples were thawed at 37ºC for 30 s and added to 1.5 mL of 45% Percoll and 1.5 mL of 90% Percoll in a 15-mL conical plastic tube. To separate HMS and LMS, sperm suspensions were stacked on top of the gradient and centrifuged at 700 x g for 10 min. The morphologically abnormal spermatozoa, seminal plasma, and extender were recovered from the top layer consisting of 45% Percoll; LMS were recovered from the 45–90% Percoll interface; and HMS were recovered from the bottom layer consisting of 90% Percoll. CASA was used to assess sperm quality according to the World Health Organization (WHO) standards.
HostingRepository	iProX
AnnounceDate	2024-02-07
AnnouncementXML	Submission_2024-02-08_04:28:54.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renzheng Zhang
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-08 04:28:31	ID requested
⏵ 1	2024-02-08 04:28:55	announced

Dataset with its publication pending

submitter keyword: Bull, Sperm, Proteomics

Renzheng Zhang
contact affiliation	Qingdao Agricultural University
contact email	zhangrenzheng51@163.com
lab head
Renzheng Zhang
contact affiliation	Qingdao Agricultural University
contact email	zhangrenzheng51@163.com
dataset submitter

iProX dataset URI