PXD049262

PXD049262 is an original dataset announced via ProteomeXchange.

Title	- Quantitative proteomics pointed out the possible role of the sox system in the interplay between sulphur and arsenic metabolism in the phototroph Halorhodospira halophila
Description	Study of the photosynthetic metabolism of purple sulphur bacteria Halorhodospira halophila by analysing the proteome of the bacteria cultivated with various sulphur compounds. In this way for the first time a comprehensive sulphur-oxidative photosynthetic network of an Halorhodospiraceaen is presented. As some Ectothiorhodospiraceae have been shown to use arsenite as photosynthetic electron donor, We performed a mass spectrometry-based whole proteome analysis with label-free quantification of H. halophila photoheterotrophically grown in the presence of various inorganic reduced sulphur compounds and in the concomitant presence of sulphide and arsenite, in order to identify proteins (enzymes and electron carriers) involved in its photosynthetic metabolism. Quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced in this latter growth condition as are two electron carriers, the cytochrome c551/c5 and PioC/HiPIP III. Taken together, these results argue in favour of a chemically-formed thioarsenate oxidized by the Sox system, with c551/c5 and PioC/HiPIP III funnelling electrons towards the Reaction Centre.
HostingRepository	PRIDE
AnnounceDate	2024-04-09
AnnouncementXML	Submission_2024-04-09_04:14:24.341.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Halorhodospira halophila; NCBI TaxID: 1053; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-08 03:48:23	ID requested
⏵ 1	2024-04-09 04:14:24	announced

Dataset with its publication pending

submitter keyword: arsenic metabolism, sulphur oxidizing bacteria, proteomics, thioarsenic, anaerobic photosynthesis,sulphur metabolism, Halorhodospira halophila, Ectothiorhodospiraceae

Luc Camoin
contact affiliation	Marseille Protéomique - Centre de Recherche en Cancérologie de Marseille - INSERM U1068 - CNRS BIP UMR 7281
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049262
     1. Label: PRIDE project
     2. Name: - Quantitative proteomics pointed out the possible role of the sox system in the interplay between sulphur and arsenic metabolism in the phototroph Halorhodospira halophila