PXD049255

PXD049255 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Deep Proteome Turnover of Human iPSC-derived Neurons
Description	Proteostasis involves a dynamic network of biological pathways that regulate protein synthesis, maintenance, and degradation. As postmitotic cells, neurons are particularly sensitive to environmental changes, and dysfunction in cellular proteostasis can lead to an accumulation of aggregated and misfolded proteins. However, how proteins turnover on a global scale in human neurons is not well understood. In this study, we systematically improved a dynamic SILAC proteomic approach to enable a deep and accurate measurement of protein turnover in human induced pluripotent stem cell (iPSC)-derived cholinergic spinal motor and glutamatergic cortical neurons. Furthermore, we applied this deep proteome turnover method to evaluate how inhibiting the ubiquitin-proteasome and lysosome-autophagy pathway impacts proteostasis in iPSC-derived neurons. Using these datasets, we developed a freely available resource called Neuron Profile, an interactive website for visualizing and querying protein turnover in subcellular locations in human neurons.
HostingRepository	MassIVE
AnnounceDate	2024-11-02
AnnouncementXML	Submission_2024-11-02_07:31:06.379.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Hao
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification; unknown modification; Carbamidomethyl; Oxidation; Acetyl
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-07 16:32:04	ID requested
⏵ 1	2024-11-02 07:31:06	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: dynamic SILAC, proteostasis, Protein Turnover, Protein Half-life, iPSC, neurons, spinal motor neurons, cortical neurons, glutamatergic neurons, cholinergic neurons, NeuronProfile, proteasome, autophagy, bafilomycin, epoxomicin

submitter keyword: dynamic SILAC, proteostasis, Protein Turnover, Protein Half-life, iPSC, neurons, spinal motor neurons, cortical neurons, glutamatergic neurons, cholinergic neurons, NeuronProfile, proteasome, autophagy, bafilomycin, epoxomicin

Contact List

Ling Hao
contact affiliation	The George Washington University
contact email	linghao@gwu.edu
lab head
Ling Hao
contact affiliation	University of Maryland
contact email	haolab.19@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094033/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094033/