PXD049252

PXD049252 is an original dataset announced via ProteomeXchange.

Title	Redox proteomics optimized protocol applyed to melanoma cells
Description	Cancer development and progression is intimately related with post-translational protein modifications, e.g., highly reactive thiol moiety of cysteines enables structural rearrangements resulting in redox biological switches. In this context, redox proteomics emerge as a fundamental tool to identify and quantify redox-sensitive proteins and to understand redox mechanisms behind thiol modifications. Given the great variability in redox proteomics protocols, problems including decreased resolution of peptides and low protein amounts even after the enrichment steps may occur. Considering the biological importance of thiol’s oxidation in melanoma, we adapted the biotin-switch assay technique for melanoma cells in order to overcome limitations of the traditional method.
HostingRepository	PRIDE
AnnounceDate	2024-02-28
AnnouncementXML	Submission_2024-02-28_05:49:40.057.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michel Batista
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	N-ethylmaleimide derivatized cysteine; biotinylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-07 12:04:45	ID requested
⏵ 1	2024-02-28 05:49:40	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, redox proteomics, melanoma cells

Glaucia Regina Martinez
contact affiliation	Department of Biochemistry and Molecular Biology - Postgraduate Program in Sciences (Biochemistry) - UFPR
contact email	grmartinez@ufpr.br
lab head
Michel Batista
contact affiliation	Fiocruz
contact email	batista.mic@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD049252

PRIDE project URI

• PRIDE
  1. PXD049252
     1. Label: PRIDE project
     2. Name: Redox proteomics optimized protocol applyed to melanoma cells