PXD049232

PXD049232 is an original dataset announced via ProteomeXchange.

Title	Structural characterization of the oligomerization of full-length Hantaan virus polymerase into symmetric dimers and hexamers
Description	Hantaan virus is a dangerous human pathogen whose segmented negative-stranded RNA genome is replicated and transcribed by a virally-encoded multi-functional polymerase. To describe the complete cryo-electron microscopy structure of Hantaan virus polymerase in several oligomeric forms, a full-length wild-type (WT) HTNV-L construct comprising an N-terminal his-tag was expressed in Hi5 insect cells and subsequently purified. Through a combination of size-exclusion chromatography and mass photometry analysis, the presence of distinct HTNV-L oligomers was observed. In buffers containing 250mM NaCl, several species were automatically detected including monomers (53%), dimers (17%), higher molecular weight oligomers up to hexamers (2%) and an unidentified 150kDa protein (18%). To characterize the proteins present in the 150kDa band, a bottom-up mass spectrometry (MS)-based proteomic analysis was performed. For this, proteins present in the 150 kDa band were in-gel digested with trypsin before analysis by nano liquid chromatography coupled to MS/MS. Obtained result revealed that the most abundant protein in this band was HTNV-L. The identified peptides were covering the entire HTNV-L sequence, suggesting that the 150kDa band may contain different cleaved forms of HTNV-L co-migrating in this particular SDS-PAGE gel band.
HostingRepository	PRIDE
AnnounceDate	2024-02-07
AnnouncementXML	Submission_2024-02-07_03:04:36.932.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049232
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Hantaan virus 76-118; NCBI TaxID: 11602; scientific name: Trichoplusia ni; NCBI TaxID: 7111;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-07 02:44:40	ID requested
⏵ 1	2024-02-07 03:04:37	announced

10.6019/PXD049232;

submitter keyword: Hantaan virus, structure, polymerase, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049232
     1. Label: PRIDE project
     2. Name: Structural characterization of the oligomerization of full-length Hantaan virus polymerase into symmetric dimers and hexamers