PXD049227
PXD049227 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The kinase NEK6 positively regulates LSD1 activity and accumulation in local chromatin sub-compartments (CSCs) |
Description | LSD1 is involved in multiple essential roles in mammalian biology and various modes of LSD1 regulation are described ranging from interaction with specific coregulators to distinct post translational modifications. Here we show that NEK6 positively influences LSD1 activity and we observe a strong colocalization of NEK6 and LSD1 at distinct chromatin sub-compartments (CSCs). We further demonstrate that LSD1 is a substrate for NEK6-mediated phosphorylation at the highly conserved, but intrinsic disordered region (IDR) of LSD1, which shows phase separation behaviour in-vitro and in cells. Furthermore, the IDR of LSD1 is important for LSD1 activity and functions to co-compartmentalize histone tails. Our data suggest that LSD1 and NEK6 form distinct CSCs in part through the formation of phase-separated condensates. The subsequent phosphorylation of LSD1 by NEK6 within these CSCs aids to concentrate more LSD1 leading to co-compartmentalisation of histone substrates, which is imperative for dynamic and robust control of transcription. |
HostingRepository | jPOST |
AnnounceDate | 2024-10-27 |
AnnouncementXML | Submission_2024-10-28_01:36:40.566.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jürgen Eirich |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-02-07 01:21:11 | ID requested | |
⏵ 1 | 2024-10-28 01:36:40 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: phosphorylation |
Contact List
Iris Finkemeier | |
---|---|
lab head | |
Jürgen Eirich | |
contact affiliation | University of Münster - WWU - Institute for Biology and Biotechnology of Plants |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
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