PXD049227

PXD049227 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The kinase NEK6 positively regulates LSD1 activity and accumulation in local chromatin sub-compartments (CSCs)
Description	LSD1 is involved in multiple essential roles in mammalian biology and various modes of LSD1 regulation are described ranging from interaction with specific coregulators to distinct post translational modifications. Here we show that NEK6 positively influences LSD1 activity and we observe a strong colocalization of NEK6 and LSD1 at distinct chromatin sub-compartments (CSCs). We further demonstrate that LSD1 is a substrate for NEK6-mediated phosphorylation at the highly conserved, but intrinsic disordered region (IDR) of LSD1, which shows phase separation behaviour in-vitro and in cells. Furthermore, the IDR of LSD1 is important for LSD1 activity and functions to co-compartmentalize histone tails. Our data suggest that LSD1 and NEK6 form distinct CSCs in part through the formation of phase-separated condensates. The subsequent phosphorylation of LSD1 by NEK6 within these CSCs aids to concentrate more LSD1 leading to co-compartmentalisation of histone substrates, which is imperative for dynamic and robust control of transcription.
HostingRepository	jPOST
AnnounceDate	2024-10-27
AnnouncementXML	Submission_2024-10-28_01:36:40.566.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jürgen Eirich
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-07 01:21:11	ID requested
⏵ 1	2024-10-28 01:36:40	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: phosphorylation

submitter keyword: phosphorylation

Contact List

Iris Finkemeier
lab head
Jürgen Eirich
contact affiliation	University of Münster - WWU - Institute for Biology and Biotechnology of Plants
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002480/