PXD049224 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | NME3 is a gatekeeper for DRP1-dependent mitophagy in hypoxia |
| Description | NME3 is a member of the nucleoside diphosphate kinase (NDPK) family localized on the mitochondrial outer membrane (MOM). Here, we report a role of NME3 in hypoxia-induced mitophagy dependent on its active site phosphohistidine but not the NDPK function. Mice carrying a knock-in mutation in the Nme3 gene disrupting NME3 active site histidine phosphorylation are vulnerable to ischemia/reperfusion-induced infarction and develop abnormalities in cerebellar function. Our mechanistic analysis reveals that hypoxia-induced phosphatidic acid (PA) on mitochondria is essential for mitophagy and the interaction of DRP1 with NME3. The PA binding function of MOM-localized NME3 is required for hypoxia-induced mitophagy. Further investigation demonstrates that the interaction with active NME3 prevents DRP1 susceptibility to MUL1-mediated ubiquitination, thereby allowing a sufficient amount of active DRP1 to mediate mitophagy. Furthermore, MUL1 overexpression suppresses hypoxia-induced mitophagy, which is reversed by co-expression of ubiquitin-resistant DRP1 mutant or histidine phosphorylatable NME3. Thus, the site-specific interaction with active NME3 provides DRP1 a microenvironment for stabilization to proceed the segregation process in mitophagy. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-23 |
| AnnouncementXML | Submission_2026-02-22_16:14:36.755.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yi-Ju Chen |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-02-06 23:50:46 | ID requested | |
| ⏵ 1 | 2026-02-22 16:14:37 | announced | |
Publication List
| Chen CW, Su C, Huang CY, Huang XR, Cuili X, Chao T, Fan CH, Ting CW, Tsai YW, Yang KC, Yeh TY, Hsieh ST, Chen YJ, Feng Y, Hunter T, Chang ZF, NME3 is a gatekeeper for DRP1-dependent mitophagy in hypoxia. Nat Commun, 15(1):2264(2024) [pubmed] |
| 10.1038/s41467-024-46385-7; |
Keyword List
| submitter keyword: hypoxia,NME3, mitochondria, mouse, LC-MS/MS |
Contact List
| Zee-Fen Chang |
|---|
| contact affiliation | Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan |
| contact email | zfchang@ntu.edu.tw |
| lab head | |
| Yi-Ju Chen |
|---|
| contact affiliation | National Yang Ming Chiao Tung University |
| contact email | tp7249@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD049224 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049224
- Label: PRIDE project
- Name: NME3 is a gatekeeper for DRP1-dependent mitophagy in hypoxia
