PXD049219

PXD049219 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based proteomic analysis of the liprin-α1 interactome in MIN6 cells
Description	Accumulating evidence suggests that insulin secretion in pancreatic β cells is regulated by mechanisms analogous to synaptic vesicle release at the neuronal presynaptic active zone (AZ). Neurotransmitter release is tightly regulated in magnitude, space and time by a presynaptic macromolecular complex including scaffold proteins liprin, ELKS, RIM and RIM-BP, to ensure precise control of synaptic responses. This presynaptic complex tethers, docks and locally controls the fusion of synaptic vesicles at the AZ. β-cells express many of the same neuronal presynaptic scaffold proteins including liprin-α1, however, no examination into the existence of a β cell presynaptic-like complex has been done. Here, we performed the first comprehensive interactome analysis of liprin-α1 in MIN6 cells using co-immunoprecipitation coupled to mass spectrometry-based proteome analysis to identify liprin-α1 binding partners and probe the existence of a wider β cell presynaptic-like complex. We identified a broad range of liprin-α1 interacting proteins in both basal (low glucose) and stimulated (high glucose) conditions. Key among these were the synaptic proteins liprin-α1,2, liprin-β1, GIT1 and LAR, supporting a β cell presynaptic-like complex. Other proteins of interest included β2 syntrophin, a regulator of insulin granule mobility, the microtubule organising proteins Mtcl1 and Mtcl3, as well as protein phosphatase 2A complexes and the functionally related 14-3-3 isoforms involved in phospho-protein dependent binding. Together, these data indicate liprin-α1 is central to a wider presynaptic-like complex that is the focus for phosphorylation and structurally links insulin granule positioning and cytoskeletal dynamics.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:12:48.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Larance
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-06 20:04:35	ID requested
⏵ 1	2026-02-08 16:12:49	announced

10.1083/jcb.202410210;

Deng K, Sun K, Hallahan N, Gan WJ, Cielesh M, Mahyad B, Kebede MA, Larance M, Thorn P, 1 clusters spatially localize insulin granule fusion. J Cell Biol, 224(10):(2025) [pubmed]

submitter keyword: presynaptic complex,pancreatic β cell, MIN6, active zone, insulin exocytosis

Mark Larance
contact affiliation	Charles Perkins Centre, University of Sydney, Camperdown, NSW, Australia
contact email	mark.larance@sydney.edu.au
lab head
Mark Larance
contact affiliation	The University of Sydney
contact email	mark.larance@sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049219
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based proteomic analysis of the liprin-α1 interactome in MIN6 cells