PXD049213

PXD049213 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Pleurotus ostreatus grown on glucose and xy-lose mixtures in submerged fermentation
Description	Pleurotus ostreatus, an edible white-rot fungus of great commercial and nutritional value, grows by metabolizing mainly glucose and xylose, the two major sugars in lignocellulosic biomass. In this study, a comparative proteomic analysis of P. ostreatus grown in submerged fermentation on medium with glucose, xylose and mixtures of them as carbon sources was conducted. In the same conditions, the metabolic response of the fungus was evaluated in the production of the main nutritional components of the fungus such as proteins, lipids, intracellular and extracellular polysaccharides. Xylose is found to up-regulate the tricarboxylic acid cycle whereas glucose upregulates the glycolysis cycle. An analysis of the upregulated proteins through the STRING database revealed that xylose upregulates mostly proteins related to amino acid biosynthesis. Leucine, Valine and Isoleucine biosynthesis pathways were found to be the most triggered pathway. These findings provide new insights into the proteomic and metabolic response of the fungus to the major sugars of lignocellulosic biomass. Based on these results, the metabolism of P. ostreatus could be turned towards a specific biological process using a suitable substrate. In addition, from proteomic analysis, strategies for genetic engineering of the strain could be adopted aiming to enhance protein expression, secretion and biotechnological-related pathways of the strain, a very constructive tool for the food industry.
HostingRepository	PRIDE
AnnounceDate	2024-11-19
AnnouncementXML	Submission_2024-11-19_07:48:49.131.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Pleurotus ostreatus; NCBI TaxID: 5322;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-06 08:07:23	ID requested
⏵ 1	2024-11-19 07:48:49	announced

Dataset with its publication pending

submitter keyword: Pleurotus ostreatus

proteomics

submerged cultivation

glucose

xylose

proteins

IPS

EPS

metabolic pathways

Petros Katapodis
contact affiliation	Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece
contact email	pkatapo@uoi.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049213
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Pleurotus ostreatus grown on glucose and xy-lose mixtures in submerged fermentation