PXD049195

PXD049195 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Extensive structural rearrangement of intraflagellar transport trains underpins bidirectional cargo transport
Description	Bidirectional transport in cilia is carried out by polymers of the IFTA and IFTB protein complexes, referred to as anterograde and retrograde IFT “trains”. Anterograde trains deliver cargoes from the cell to the ciliary tip, then remodel into retrograde trains for cargo export. We set out to understand how the structure of these trains changes at the tip, and thus how the same IFT complexes can perform opposing transport roles. We use cryo-electron tomography, subtomogram averaging and in-situ cross-linking mass spectrometry to determine the structure of retrograde IFT trains, and compare with the known anterograde train structure. We show that the retrograde train is a two-fold symmetric polymer organised around a central thread of IFTA complexes. We conclude that the structural differences between anterograde and retrograde trains can only be achieved through de novo polymerisation of the retrograde train from individual IFTA/B complexes. Finally we describe how conformational changes to cargo binding sites allow for unidirectional cargo transport in a bidirectional system.
HostingRepository	jPOST
AnnounceDate	2024-08-21
AnnouncementXML	Submission_2024-08-21_04:07:38.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Samuel Lacey
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification; unknown modification; unknown modification; unknown modification; unknown modification; unknown modification
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-06 00:25:43	ID requested
⏵ 1	2024-08-21 04:07:39	announced

Publication List

Lacey SE, Graziadei A, Pigino G, Extensive structural rearrangement of intraflagellar transport trains underpins bidirectional cargo transport. Cell, 187(17):4621-4636.e18(2024) [pubmed]

Lacey SE, Graziadei A, Pigino G, Extensive structural rearrangement of intraflagellar transport trains underpins bidirectional cargo transport. Cell, 187(17):4621-4636.e18(2024) [pubmed]

Keyword List

submitter keyword: crosslinking mass spectrometry, chlamydomonas reinhardtii, cilia, intraflagellar transport

submitter keyword: crosslinking mass spectrometry, chlamydomonas reinhardtii, cilia, intraflagellar transport

Contact List

Gaia Pigino
lab head
Samuel Lacey
contact affiliation	Human Technopole
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002488/