PXD049176

PXD049176 is an original dataset announced via ProteomeXchange.

Title	Circadian regulation of macromolecular complex turnover and proteome renewal
Description	Although costly to maintain, protein homeostasis is indispensable for normal cellular function and long-term health. In mammalian cells and tissues, daily variation in global protein synthesis has been observed, but its utility and consequences for proteome integrity are not fully understood. Using several different pulse-labelling strategies, here we gain direct insight into the relationship between protein synthesis and abundance proteome- wide. We show that protein degradation varies in-phase with protein synthesis, facilitating rhythms in turnover rather than abundance. This results in daily consolidation of proteome renewal whilst minimising changes in composition. Coupled rhythms in synthesis and turnover are especially salient to the assembly of macromolecular protein complexes, particularly the ribosome, the most abundant species of complex in the cell. Daily turnover and proteasomal degradation rhythms render cells and mice more sensitive to proteotoxic stress at specific times of day, potentially contributing to daily rhythms in the efficacy of proteasomal inhibitors against cancer. Our findings suggest that circadian rhythms function to minimise the bioenergetic cost of protein homeostasis through temporal consolidation of protein turnover.
HostingRepository	PRIDE
AnnounceDate	2024-05-16
AnnouncementXML	Submission_2024-05-16_05:49:27.748.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sew Peak-Chew
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-05 04:22:45	ID requested
⏵ 1	2024-05-16 05:49:28	announced

10.1038/S44318-024-00121-5;

submitter keyword: circardian rhythms,Protein homoestasis, pSILAC_TMT, complex turnover

Andrew Beale
contact affiliation	MRC-Laboratory of Molecular Biology Francis Crick Avenus Cambridge CB20QH
contact email	abeale@mrc-lmb.cam.ac.uk
lab head
Sew Peak-Chew
contact affiliation	MRC-LMB
contact email	spc@mrc-lmb.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049176
     1. Label: PRIDE project
     2. Name: Circadian regulation of macromolecular complex turnover and proteome renewal