PXD049153

PXD049153 is an original dataset announced via ProteomeXchange.

Title	Bioorthogonally activated reactive species for target identification
Description	A target identification platform derived from the bioorthogonal activation of reactive species is described. We explore the reactivity of halogenated enamine N-oxides and report that the previously undisclosed α,γ-halogenated enamine N-oxides can be reduced biooorthogonally by diboron reagents to produce highly electrophilic α,β-unsaturated haloiminium ions suitable for labeling a range of amino acid residues on proteins in a 1,2- or 1,4-fashion. Affinity labeling reagents bearing this motif enable ligand-directed protein modification and afford highly sensitive and selective target identification in unbiased chemoproteomics experiments. Target identification is supported in both cell lysate and live cells.
HostingRepository	PRIDE
AnnounceDate	2024-04-03
AnnouncementXML	Submission_2024-04-03_10:48:01.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Surached Siriwongsup
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-04 09:21:50	ID requested
⏵ 1	2024-04-03 10:48:01	announced

10.1016/J.CHEMPR.2024.03.002;

submitter keyword: AP-MS,Bioorthogonal, chemoproteomics, target ID

Justin Kim
contact affiliation	1 Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02215, United States 2 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, United States
contact email	Justin_Kim@dfci.harvard.edu
lab head
Surached Siriwongsup
contact affiliation	Dana-Farber Cancer Institute
contact email	surached_siriwongsup@dfci.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD049153

PRIDE project URI

• PRIDE
  1. PXD049153
     1. Label: PRIDE project
     2. Name: Bioorthogonally activated reactive species for target identification