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PXD049147

PXD049147 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe TET-Sall4-BMP regulatory axis controls craniofacial cartilage development
DescriptionCraniofacial microsomia (CFM) is a congenital defect that usually results from aberrant development of embryonic pharyngeal arches. However, the molecular basis of CFM pathogenesis is largely unknown. Here, we employ the zebrafish model to investigate mechanisms of CFM pathogenesis. In early embryos, tet2 and tet3 are essential for pharyngeal cartilage development. Single-cell RNA-sequencing reveals that loss of Tet2/3 impaired chondrocyte differentiation due to insufficient BMP signaling. Moreover, biochemical and genetic evidence reveals that the sequence-specific 5mC/5hmC-binding protein, Sall4, binds the promoter of bmp4 to activate bmp4 expression and control pharyngeal cartilage development. Mechanistically, Sall4 directs co-phase separation of Tet2/3 with Sall4 to form condensates that mediate 5mC oxidation on the bmp4 promoter, thereby promoting bmp4 expression and enabling sufficient BMP signaling. These findings suggest the TET-BMP-Sall4 regulatory axis is critical for pharyngeal cartilage development. Collectively, our study provides novel insights into understanding craniofacial development and CFM pathogenesis.
HostingRepositoryiProX
AnnounceDate2024-02-02
AnnouncementXMLSubmission_2024-10-12_00:38:45.852.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWeigang Wang
SpeciesList scientific name: Danio rerio; NCBI TaxID: 7955;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-03 19:58:14ID requested
12024-02-03 19:58:26announced
22024-10-12 00:38:46announced2024-10-12: Update information.
Publication List
Wang W, Yang N, Wang L, Zhu Y, Chu X, Xu W, Li Y, Xu Y, Gao L, Zhang B, Zhang G, Sun Q, Wang W, Wang Q, Zhang W, Chen D, The TET-Sall4-BMP regulatory axis controls craniofacial cartilage development. Cell Rep, 43(3):113873(2024) [pubmed]
Keyword List
submitter keyword: craniofacial microsomia, craniofacial development, TET proteins, Phase separation
Contact List
Dahua Chen
contact affiliationInstitute of Biomedical Research, Yunnan University
contact emailchendh@ynu.edu.cn
lab head
Weigang Wang
contact affiliationInstitute of Biomedical Research, Yunnan University
contact emailwangweigang@itc.ynu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI