PXD049147
PXD049147 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The TET-Sall4-BMP regulatory axis controls craniofacial cartilage development |
Description | Craniofacial microsomia (CFM) is a congenital defect that usually results from aberrant development of embryonic pharyngeal arches. However, the molecular basis of CFM pathogenesis is largely unknown. Here, we employ the zebrafish model to investigate mechanisms of CFM pathogenesis. In early embryos, tet2 and tet3 are essential for pharyngeal cartilage development. Single-cell RNA-sequencing reveals that loss of Tet2/3 impaired chondrocyte differentiation due to insufficient BMP signaling. Moreover, biochemical and genetic evidence reveals that the sequence-specific 5mC/5hmC-binding protein, Sall4, binds the promoter of bmp4 to activate bmp4 expression and control pharyngeal cartilage development. Mechanistically, Sall4 directs co-phase separation of Tet2/3 with Sall4 to form condensates that mediate 5mC oxidation on the bmp4 promoter, thereby promoting bmp4 expression and enabling sufficient BMP signaling. These findings suggest the TET-BMP-Sall4 regulatory axis is critical for pharyngeal cartilage development. Collectively, our study provides novel insights into understanding craniofacial development and CFM pathogenesis. |
HostingRepository | iProX |
AnnounceDate | 2024-02-02 |
AnnouncementXML | Submission_2024-10-12_00:38:45.852.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Weigang Wang |
SpeciesList | scientific name: Danio rerio; NCBI TaxID: 7955; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-02-03 19:58:14 | ID requested | |
1 | 2024-02-03 19:58:26 | announced | |
⏵ 2 | 2024-10-12 00:38:46 | announced | 2024-10-12: Update information. |
Publication List
Wang W, Yang N, Wang L, Zhu Y, Chu X, Xu W, Li Y, Xu Y, Gao L, Zhang B, Zhang G, Sun Q, Wang W, Wang Q, Zhang W, Chen D, The TET-Sall4-BMP regulatory axis controls craniofacial cartilage development. Cell Rep, 43(3):113873(2024) [pubmed] |
Keyword List
submitter keyword: craniofacial microsomia, craniofacial development, TET proteins, Phase separation |
Contact List
Dahua Chen | |
---|---|
contact affiliation | Institute of Biomedical Research, Yunnan University |
contact email | chendh@ynu.edu.cn |
lab head | |
Weigang Wang | |
contact affiliation | Institute of Biomedical Research, Yunnan University |
contact email | wangweigang@itc.ynu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |