PXD049147

PXD049147 is an original dataset announced via ProteomeXchange.

Title	The TET-Sall4-BMP regulatory axis controls craniofacial cartilage development
Description	Craniofacial microsomia (CFM) is a congenital defect that usually results from aberrant development of embryonic pharyngeal arches. However, the molecular basis of CFM pathogenesis is largely unknown. Here, we employ the zebrafish model to investigate mechanisms of CFM pathogenesis. In early embryos, tet2 and tet3 are essential for pharyngeal cartilage development. Single-cell RNA-sequencing reveals that loss of Tet2/3 impaired chondrocyte differentiation due to insufficient BMP signaling. Moreover, biochemical and genetic evidence reveals that the sequence-specific 5mC/5hmC-binding protein, Sall4, binds the promoter of bmp4 to activate bmp4 expression and control pharyngeal cartilage development. Mechanistically, Sall4 directs co-phase separation of Tet2/3 with Sall4 to form condensates that mediate 5mC oxidation on the bmp4 promoter, thereby promoting bmp4 expression and enabling sufficient BMP signaling. These findings suggest the TET-BMP-Sall4 regulatory axis is critical for pharyngeal cartilage development. Collectively, our study provides novel insights into understanding craniofacial development and CFM pathogenesis.
HostingRepository	iProX
AnnounceDate	2024-02-02
AnnouncementXML	Submission_2024-10-12_00:38:45.852.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weigang Wang
SpeciesList	scientific name: Danio rerio; NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-03 19:58:14	ID requested
1	2024-02-03 19:58:26	announced
⏵ 2	2024-10-12 00:38:46	announced	2024-10-12: Update information.

Wang W, Yang N, Wang L, Zhu Y, Chu X, Xu W, Li Y, Xu Y, Gao L, Zhang B, Zhang G, Sun Q, Wang W, Wang Q, Zhang W, Chen D, The TET-Sall4-BMP regulatory axis controls craniofacial cartilage development. Cell Rep, 43(3):113873(2024) [pubmed]

submitter keyword: craniofacial microsomia, craniofacial development, TET proteins, Phase separation

Dahua Chen
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	chendh@ynu.edu.cn
lab head
Weigang Wang
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	wangweigang@itc.ynu.edu.cn
dataset submitter

iProX dataset URI