PXD049140

PXD049140 is an original dataset announced via ProteomeXchange.

Title	[HPP-2.0] Integrated and high-throughput approach for sensitive analysis of tyrosine phosphoproteome
Description	In this study, we developed a 96-well microplate-based approach to integrate all the sample preparation steps starting from cell culture to MS-compatible pTyr peptide enrichment in three consecutive 96-well microplates. By assembling engineered SH2 domain onto microplate, non-specific adsorption of phosphopeptides is greatly reduced, which allows us to remove the Ti-IMAC purification and three C18 desalting steps, and therefore greatly simplify the entire pTyr peptide enrichment workflow, especially when processing a large number of samples. Starting with 96-well microplate-cultured cells, our approach could enrich 21% more pTyr sites than the traditional serial pTyr enrichment approach and showed good sensitivity and reproducibility in the range of 200 ng to 200 μg peptides. Importantly, we applied this approach to profile tyrosine kinase inhibitors-mediated EGFR signaling pathway and could well differentiate the distinct response of different pTyr sites. Collectively, the integrated 96-well microplate-based
HostingRepository	iProX
AnnounceDate	2024-02-03
AnnouncementXML	Submission_2024-10-12_00:26:10.992.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruijun Tian
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-03 19:43:02	ID requested
1	2024-02-03 19:43:29	announced
⏵ 2	2024-10-12 00:26:20	announced	2024-10-12: Update information.

Kong Q, Weng Y, Zheng Z, Chen W, Li P, Cai Z, Tian R, Integrated and High-Throughput Approach for Sensitive Analysis of Tyrosine Phosphoproteome. Anal Chem, 94(40):13728-13736(2022) [pubmed]

submitter keyword: high throughput, phosphotyrosine site

Ruijun Tian
contact affiliation	Southern University of Science and Technology
contact email	tianrj@sustech.edu.cn
lab head
Ruijun Tian
contact affiliation	Southern University of Science and Technology
contact email	13667356941@163.com
dataset submitter

iProX dataset URI