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PXD049140

PXD049140 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title[HPP-2.0] Integrated and high-throughput approach for sensitive analysis of tyrosine phosphoproteome
DescriptionIn this study, we developed a 96-well microplate-based approach to integrate all the sample preparation steps starting from cell culture to MS-compatible pTyr peptide enrichment in three consecutive 96-well microplates. By assembling engineered SH2 domain onto microplate, non-specific adsorption of phosphopeptides is greatly reduced, which allows us to remove the Ti-IMAC purification and three C18 desalting steps, and therefore greatly simplify the entire pTyr peptide enrichment workflow, especially when processing a large number of samples. Starting with 96-well microplate-cultured cells, our approach could enrich 21% more pTyr sites than the traditional serial pTyr enrichment approach and showed good sensitivity and reproducibility in the range of 200 ng to 200 μg peptides. Importantly, we applied this approach to profile tyrosine kinase inhibitors-mediated EGFR signaling pathway and could well differentiate the distinct response of different pTyr sites. Collectively, the integrated 96-well microplate-based
HostingRepositoryiProX
AnnounceDate2024-02-03
AnnouncementXMLSubmission_2024-10-12_00:26:10.992.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRuijun Tian
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-03 19:43:02ID requested
12024-02-03 19:43:29announced
22024-10-12 00:26:20announced2024-10-12: Update information.
Publication List
Kong Q, Weng Y, Zheng Z, Chen W, Li P, Cai Z, Tian R, Integrated and High-Throughput Approach for Sensitive Analysis of Tyrosine Phosphoproteome. Anal Chem, 94(40):13728-13736(2022) [pubmed]
Keyword List
submitter keyword: high throughput, phosphotyrosine site
Contact List
Ruijun Tian
contact affiliationSouthern University of Science and Technology
contact emailtianrj@sustech.edu.cn
lab head
Ruijun Tian
contact affiliationSouthern University of Science and Technology
contact email13667356941@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI