PXD049138

PXD049138 is an original dataset announced via ProteomeXchange.

Title	Chitosan oligosaccharide exhibits potential as a drug candidate for cholera treatment and prevention by blocking the ChsR-mediated virulence pathway
Description	DNA pulldown assays were performed as previously described 43. Briefly, the biotin-labeled DNA fragment containing the promoter region of chsR was amplified from Vibrio cholerae O1 El Tor strain E12382 genomic DNA. The biotinylated bait DNA was bound to streptavidin-coated Dynabeads (catalog no. 11205D; Invitrogen), followed by incubation with crude extracts obtained from Vibrio cholerae O1 El Tor WT cells. After washing extensively with the nonspecific competitor poly (dI-dC) at a low salt concentration, bound proteins were released by elution buffer containing 500 mM NaCl. The eluted proteins were separated by SDS‒PAGE and stained with Coomassie brilliant blue. Proteins were excised from the gel and analyzed by matrix-assisted laser desorption ionization–time-of-flight tandem mass spectrometry (MALDI-TOF MS/MS) analysis after tryptic digestion. Sequence and peptide fingerprint data were analyzed using the NCBI database.
HostingRepository	iProX
AnnounceDate	2024-02-02
AnnouncementXML	Submission_2024-02-03_19:38:04.441.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jialin Wu
SpeciesList	scientific name: Vibrio cholerae O1 biovar El Tor str. N16961; NCBI TaxID: 243277;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-03 19:37:53	ID requested
⏵ 1	2024-02-03 19:38:04	announced

Dataset with its publication pending

submitter keyword: Vibrio cholerae, LacI-type regulator, pathogenicity, chitosan oligosaccharide, microspheres

yutao Liu
contact affiliation	Nankai University
contact email	liuyutao623@nankai.edu.cn
lab head
Jialin Wu
contact affiliation	Nankai University
contact email	wujialinxuan@163.com
dataset submitter

iProX dataset URI