PXD049128

PXD049128 is an original dataset announced via ProteomeXchange.

Title	Mycobacterium smegmatis Growth Using Nitrate as a Nitrogen Source LC-MSMS
Description	PII proteins are signal transduction proteins that belong to a widely distributed family involved in modulating various bacterial metabolisms. These homotrimeric proteins carry a flexible loop, known as the T-loop, whose conformation changes upon recognition of key metabolites such as ADP, ATP, and 2-oxoglutarate. PII proteins interact with various partners to primarily regulate nitrogen pathways, and in some organisms, they can also control carbon metabolism by interacting with the biotin carboxyl carrier protein (BCCP), a critical component of the acetyl-CoA carboxylase (ACC) enzyme complex, inhibiting its activity and consequently reducing fatty acid biosynthesis. In mycobacteria, only one PII protein has been identified; however, its physiological role remains unknown. In this study, we constructed a M. smegmatis deletion mutant, ∆MsPII, which exhibited growth deficiency when nitrate and nitrite were the sole nitrogen sources, leading to nitrite accumulation in the culture supernatant.
HostingRepository	PRIDE
AnnounceDate	2024-03-15
AnnouncementXML	Submission_2024-03-15_11:51:27.606.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049128
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Delfina Ensinck
SpeciesList	scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-02 12:19:46	ID requested
⏵ 1	2024-03-15 11:51:27	announced

10.6019/PXD049128;

10.3389/FMICB.2024.1366111;

submitter keyword: Mycobacterium smegmatis, PII protein, LC-MS/MS

Hugo Cesar Gramajo
contact affiliation	Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina
contact email	gramajo@ibr-conicet.gov.ar
lab head
Delfina Ensinck
contact affiliation	Intituto de Biologìa Celular y Molecular de Rosario-CONICET, Universidad Nacional de Rosario
contact email	delfinaensinck@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049128
     1. Label: PRIDE project
     2. Name: Mycobacterium smegmatis Growth Using Nitrate as a Nitrogen Source LC-MSMS