PXD049113

PXD049113 is an original dataset announced via ProteomeXchange.

Title	Tyrosine phosphorylation site-specific interactome of adaptor protein Shc1 explored by synthetic protein domains
Description	As a critical adaptor protein, Shc1 contains SH2 and PTB domains for scaffolding tyrosine phosphorylation-dependent protein complexes, therefore regulating growth factor receptor signaling networks. The three tyrosine phosphorylation sites (pY) on the CH1 linker region of SHC1 provide additional docking sites for recruiting additional dimension of signaling complexes. The affinity purification-mass spectrometry (AP-MS) method with synthetic phosphopeptides provides an unbiased discovery approach but is often limited by the length of synthetic peptides. Here, we chemically synthesized the CH1 region (Shc1CH1) covering 107 amino acids with the 239, 240, and 313 pY sites by multiple chemical peptide ligations. Combing with AP-MS and accurate label-free quantification, we explored the site-specific interactome of these distinct phospho-forms of Shc1 and identified the enhanced complex assembly around the double-phosphorylated Y239/204 sites. We also found that Plcg1 and Plcg2 interacted with pY313 strongly and specifically. Besides, we demonstrated that the AP-MS with the mouse Shc1CH1 probes could evaluate the subcellular EGFR/HER2 signaling in three human cell lines. Overall, long synthetic peptides assembled via peptide ligation widened the scope of peptide-based AP-MS approach for the analysis of site-specific interactomes of proteins with long phosphorylated docking sites.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:55:12.550.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiong Chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; deamidated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-02 02:39:34	ID requested
1	2024-08-15 03:30:01	announced
⏵ 2	2024-10-22 06:55:12	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: tyrosine phosphorylation, Signal transduction, Shc1,Mass spectrometry, CH1 region

Ruijun Tian
contact affiliation	Department of Chemistry, College of Science, Southern University of Science and Technology, Shenzhen 518055, China
contact email	tianrj@sustech.edu.cn
lab head
Xiong Chen
contact affiliation	Senior Research Scholar
contact email	chenx6@sustech.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049113
     1. Label: PRIDE project
     2. Name: Tyrosine phosphorylation site-specific interactome of adaptor protein Shc1 explored by synthetic protein domains