To identify novel interaction partners of PARP1, we engineered a fusion of the full-length PARP1 with the TurboID enzyme and two negative controls. The first control omitted the bait protein PARP1, excluding the potential interaction of the TurboID protein itself. However, it bears the drawback of potentially obscuring real interactions due to the high flexibility and activity of free TurboID. The second control omitted exogenous biotin, serving as a complementary measure to address this concern.