PXD049099
PXD049099 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ultrasensitive single cell proteomics based on an integrated stationary nanoliter-droplet chip |
| Description | Single-cell proteomic analyses are of fundamental importance in order to capture biological heterogeneity within complex cell systems and heterogeneous populations. Herein, we developed an automated and multiplexed sample processing workflow combining integrated stationary nanoliter-droplet chips and a commercial liquid dispensing instrument for single-cell proteomics. Utilizing this robust workflow and data independent acquisition (DIA) mode, we can profile up to 3000 protein groups in single mammalian cell with a remarkable improvement, achieving a dynamic range of 6 orders of magnitude with good reproducibility. The chip-based system was further applied in single lymphocyte analysis, with protein groups of 617 and 1089 identified at the single B and T lymphocyte, demonstrating the promising applications of single-cell insights in clinical research, especially in cancer immunology. |
| HostingRepository | iProX |
| AnnounceDate | 2024-02-01 |
| AnnouncementXML | Submission_2024-02-01_21:13:05.380.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Weijian Liang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-02-01 21:12:28 | ID requested | |
| ⏵ 1 | 2024-02-01 21:13:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: single-cell proteomics, nanoliter droplet chip, data-independent acquisition |
Contact List
| Catherine C.L. Wong | |
|---|---|
| contact affiliation | Department of Biochemistry and Biophysics, Peking University Health Science Center, Beijing, China |
| contact email | catclw321@126.com |
| lab head | |
| Weijian Liang | |
| contact affiliation | Foreseen Biotechnology(Beijing) Co., Ltd. |
| contact email | weijian.liang@foreseenbio.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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