PXD049090 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The HisRS-like domain of GCN2 is a pseudoenzyme that can bind uncharged tRNA - HX-MS Data |
Description | GCN2 is a stress response kinase that phosphorylates the translation initiation factor eIF2to inhibit general protein synthesis when activated by uncharged tRNA and stalled ribosomes. The presence of a HisRS-like domain in GCN2, normally associated with the ability to bind and aminoacylate tRNAs, led to the hypothesis that eIF2 kinase activity is regulated by the direct binding of this domain to uncharged tRNA. Here we solved the structure of the HisRS-like domain in the context of full-length GCN2 by cryoEM. Structure and function analysis shows the HisRS-like domain of GCN2 has lost tRNA charging, ATP binding, and histidine binding activity but retains the ability to bind tRNA. Hydrogen deuterium exchange mass spectrometry (HX-MS), site-directed mutagenesis and computational docking experiments support a tRNA binding model that overlaps with but is partially shifted from that employed by bona fide HisRS enzymes. These results demonstrate that the HisRS-like domain of GCN2 is a pseudoenzyme and advance our understanding of GCN2 regulation and function. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-01 |
AnnouncementXML | Submission_2024-02-01_12:32:58.618.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Frantisek Filandr |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-01 11:56:31 | ID requested | |
⏵ 1 | 2024-02-01 12:32:58 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: HDX-MS |
Contact List
David Schriemer |
contact affiliation | Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary |
contact email | dschriem@ucalgary.ca |
lab head | |
Frantisek Filandr |
contact affiliation | Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary |
contact email | frantisek.filandr@ucalgary.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049090
- Label: PRIDE project
- Name: The HisRS-like domain of GCN2 is a pseudoenzyme that can bind uncharged tRNA - HX-MS Data